Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake.
Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake.
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采用蛋白质转导结构域 TAT48 设计的 Vault 纳米粒子可增强细胞摄取。
DOI：
10.1039/c2ib20119d
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Rome,LeonardH
中科院分区：
文献类型：
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作者：
Yang,Jian;Srinivasan,Aswin;Sun,Yang;Mrazek,Jan;Shu,Zhanyong;Kickhoefer,ValerieA;Rome,LeonardH
穹窿是几乎所有真核细胞中天然存在的核糖核蛋白颗粒。它们因其形态与哥特式大教堂的拱形天花板相似而得名。这些无处不在的纳米颗粒非常丰富，根据细胞类型，在细胞质中发现104- 106个拷贝。粒子的结构壳可以从单个蛋白质（主要的穹窿蛋白质）的78个拷贝自组装。这一发现使得拱顶可以进行生物工程改造，从而产生各种新的功能和能力，旨在克服当前基因和药物递送系统所带来的许多限制。在这项研究中，我们证明，重组穹窿，加上细胞穿透肽，达特，可以迅速交付到细胞体外显着提高结合和摄取效率。这种TAT-穹窿纳米颗粒可能是一种有价值的工具，用于改善治疗药物在肿瘤部位的保留和渗透。
Vaults are naturally-occurring ribonucleoprotein particles found in nearly all eukaryotic cells. They were named for their morphological resemblance to the vaulted ceilings of gothic cathedrals. These ubiquitous nanoparticles are quite abundant with 104–106copies found in the cytoplasm depending on cell type. The structural shell of the particle can self-assemble from 78 copies of a single protein, the major vault protein. This finding has allowed vaults to be bioengineered, resulting in a variety of new functions and capabilities directed toward overcoming many limitations posed by current gene and drug delivery systems. In this study, we demonstrate that recombinant vaults, with the addition of a cell penetration peptide, TAT, can be rapidly delivered to cellsin vitrowith significantly elevated binding and uptake efficiency. This TAT-vault nanoparticle could be a valuable tool for improving the retention and penetration of therapeutic drugs at tumor sites.