Dermokine: An extensively differentially spliced gene expressed in epithelial cells
Dermokine: An extensively differentially spliced gene expressed in epithelial cells
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DOI：
10.1038/sj.jid.5700779
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发表时间：
2007-07-01
影响因子：
6.5
通讯作者：
Amegadzie, Bernard Y.
中科院分区：
文献类型：
--
作者：
Naso, Michael F.;Liang, Bailin;Amegadzie, Bernard Y.
进行的研究是为了发现在这种疾病疾病中被鉴定出的炎症性疾病中过表达的基因。 Dermokine是一种基因，最初观察到在皮肤分化层中表达的基因。它的两个主要同工型Alpha和beta是从同一基因座的不同启动子转录的，代表β亚型的C末端的alpha同工型。最近，已经确定了其他成绩单变体。在人类细胞和组织中存在这些变体的存在，鉴定出新的人类同工型以及小鼠中的伽玛同工型。 C末端截短的同工型的重组表达和分析表明该分子被眨眼糖基化并在溶液中形成多聚体。原位杂交和免疫组织化学表明，除皮肤以外，该基因在各种细胞和组织中差异表达。这些结果表明，皮肤因子基因在皮肤以外的其他组织中表达，并且该表达在转录和转录后复杂。
Studies performed to discover genes overexpressed in inflammatory diseases identified dermokine as being upregulated in such disease conditions. Dermokine is a gene that was first observed as expressed in the differentiated layers of skin. Its two major isoforms, alpha and beta, are transcribed from different promoters of the same locus, with the alpha isoform representing the C terminus of the beta isoform. Recently, additional transcript variants have been identified. Extensive in silico analysis and reverse transcriptase (RT)-PCR cloning has confirmed the existence of these variants in human cells and tissues, identified a new human isoform as well as the gamma isoform in mouse. Recombinant expression and analysis of the C-terminal truncated isoform indicate that the molecule is Winked glycosylated and forms multimers in solution. In situ hybridization and immunohistochemistry has shown that the gene is differentially expressed in various cells and tissues, other than the skin. These results show that the dermokine gene is expressed in epithelial tissues other than the skin and this expression is transciptionally and posttranscriptionally complex.