Immune response to synthetic peptides of dengue prM protein.
Immune response to synthetic peptides of dengue prM protein.
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DOI：
10.1016/s0264-410x(01)00515-1
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发表时间：
2002-03
期刊：
影响因子：
5.5
通讯作者：
S. Vázquez;M. Guzmán;G. Guillén;G. Chinea;A. B. Pérez;M. Pupo;Rosmary Rodríguez;O. Reyes;
中科院分区：
文献类型：
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作者：
S. Vázquez;M. Guzmán;G. Guillén;G. Chinea;A. B. Pérez;M. Pupo;Rosmary Rodríguez;O. Reyes;
本文报道了登革2型病毒prM蛋白5个含B细胞表位的合成肽在BALB B/c小鼠中的免疫活性。两种肽引起针对所有四种DEN血清型的中和抗体。在用五种肽中的四种免疫的小鼠中证明了病毒特异性增殖反应，证明了T细胞表位的存在。用与牛白蛋白缀合的五种肽中的三种免疫的小鼠在用DEN-2病毒攻击时显示出统计学上显著的保护水平（P<0.05）。这些结果可以构成的基础上建立的作用，登革病毒前和M抗原的抗黄病毒疫苗的发展。
The immunological activities of five synthetic peptides of the prM protein of dengue-2 (DEN-2) virus containing B cell epitopes were evaluated in BALB/c mice. Two peptides elicited neutralizing antibodies against all four DEN serotypes. Virus-specific proliferative responses were demonstrated in mice immunized with four of the five peptides, demonstrating the presence of T cell epitopes. Mice immunized with three of the five peptides conjugated with bovine albumin showed statistically significant levels (P<0.05) of protection when challenged with DEN-2 virus. These results could constitute the basis for the establishment of the role of DEN virus pre and M antigens in the development of anti-flaviviral vaccines.