Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study
Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study
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DOI：
10.1016/0022-1759(95)00289-8
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发表时间：
1996-06-21
影响因子：
2.2
通讯作者：
Reimer, CB
中科院分区：
文献类型：
--
作者：
Mestecky, J;Hamilton, RG;Reimer, CB
9个实验室采用免疫扩散、凝集、免疫组织学测定、免疫印迹以及直接结合和竞争性抑制酶免疫测定法，评价了51种对人伊加、伊加亚类、Am同种异型或分泌成分（SC）具有推定特异性的单克隆抗体（McAb）的免疫反应性和特异性。对伊加PAN（n = 24）、IgA_1（n = 7）、IgA_2（n = 3）、IgA_2 m（n = 2）、非IgA_2 m（n = 4）和SC或分泌型伊加（n = 5）进行了免疫反应性和特异性鉴定。经直接结合和竞争性抑制免疫试验，表明这些单抗与人IgG、IgM、IgD、IgE、κ和λ均不反应。有趣的是，没有鉴定出对IgA 2 m（1）具有限制性特异性的单克隆抗体。某些单克隆抗体在一个或多个试验组中表现出更高的亲和力和/或更好的性能。在这项国际合作研究中确定的伊加和SC特异性单克隆抗体有可能作为免疫化学参考试剂，以确定和定量人血清和分泌物中的单体和多聚伊加。
51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. McAbs specific for IgA PAN (n = 24), IgA1 (n = 7), IgA2 (n = 3), IgA2m(2) (n = 2), non-IgA2m(2) (n = 4) and SC or secretory IgA (n = 5) were identified that were immunoreactive and specific in the assays employed. The McAbs identified as IgA- or SC-reactive were shown to be non-reactive to human IgG, IgM, IgD, IgE, kappa and lambda by direct binding and competitive inhibition immunoassays. Interestingly, no McAbs with restricted specificity for IgA2m(1) were identified. Some McAbs displayed higher affinity and/or better performance in one or several of the assay groups. The IgA- and SC-specific McAbs identified in this international collaborative study have potential as immunochemical reference reagents to identify and quantitate monomeric and polymeric IgA in human serum and secretions.