Proteome and Ubiquitome Changes during Rose Petal Senescence
Proteome and Ubiquitome Changes during Rose Petal Senescence
复制标题
玫瑰花瓣衰老过程中蛋白质组和泛素组的变化
DOI：
10.3390/ijms20246108
复制
发表时间：
2019-12-01
影响因子：
5.6
通讯作者：
Ma, Chao
中科院分区：
文献类型：
--
作者：
Lu, Jingyun;Xu, Yanjie;Ma, Chao
花瓣衰老涉及许多生物和生化过程的程序化变化。泛素化在蛋白质降解中起关键作用，蛋白质降解是器官衰老的标志。因此，我们研究了衰老玫瑰（月季）花瓣的蛋白质组和泛素组的变化，以更好地了解它们参与花瓣衰老。在衰老花瓣中定量的3859种蛋白质中，1198种在衰老过程中上调，726种在衰老过程中下调。我们确定了2208个泛素化位点，包括298个蛋白质中的384个增加的泛素化位点和674个蛋白质中的1035个减少的泛素化位点。基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析表明，与蛋白水解和自噬途径中的肽酶相关的蛋白质在蛋白质组中富集，表明蛋白质降解和自噬在花瓣衰老中起重要作用。此外，许多转运蛋白在衰老的花瓣中积累，并且一些转运过程在泛素组中富集，表明物质的转运与花瓣衰老相关并且受泛素化调节。此外，油菜素类固醇（BR）的生物合成和信号通路的几个组件显着改变在蛋白质和泛素化水平，这意味着BR在花瓣衰老中起着重要作用。我们的数据提供了一个全面的看法，玫瑰花瓣衰老的翻译后水平。
Petal senescence involves numerous programmed changes in biological and biochemical processes. Ubiquitination plays a critical role in protein degradation, a hallmark of organ senescence. Therefore, we investigated changes in the proteome and ubiquitome of senescing rose (Rosa hybrida) petals to better understand their involvement in petal senescence. Of 3859 proteins quantified in senescing petals, 1198 were upregulated, and 726 were downregulated during senescence. We identified 2208 ubiquitinated sites, including 384 with increased ubiquitination in 298 proteins and 1035 with decreased ubiquitination in 674 proteins. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that proteins related to peptidases in proteolysis and autophagy pathways were enriched in the proteome, suggesting that protein degradation and autophagy play important roles in petal senescence. In addition, many transporter proteins accumulated in senescing petals, and several transport processes were enriched in the ubiquitome, indicating that transport of substances is associated with petal senescence and regulated by ubiquitination. Moreover, several components of the brassinosteroid (BR) biosynthesis and signaling pathways were significantly altered at the protein and ubiquitination levels, implying that BR plays an important role in petal senescence. Our data provide a comprehensive view of rose petal senescence at the posttranslational level.