Plasmids with the Cre‐recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis
Plasmids with the Cre‐recombinase and the dominant nat marker, suitable for use in prototrophic strains of Saccharomyces cerevisiae and Kluyveromyces lactis
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DOI：
10.1002/yea.696
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发表时间：
2001-03
期刊：
影响因子：
2.6
通讯作者：
H. Steensma;J. Linde
中科院分区：
文献类型：
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作者：
H. Steensma;J. Linde
描述了两种质粒，其可用于去除缺乏ura3突变的菌株中的“loxP‐markerMX‐loxP”盒。两者都含有在GAL1启动子控制下的Cre重组酶和具有显性标记nat的natMX盒，其使酵母对抗生素ClonNat具有抗性。pNatCre含有ARSH和CEN6，用于在酿酒酵母中维持。pKlNatCre还具有乳酸克鲁维酵母复制起点和着丝粒。版权所有© 2001约翰威利父子有限公司。
Two plasmids are described which can be used to remove the ‘loxP‐markerMX‐loxP’ cassettes in strains lacking the ura3 mutation. Both contain the Cre‐recombinase under control of the GAL1 promoter and the natMX cassette with the dominant marker nat, which gives yeasts resistance to the antibiotic ClonNat. pNatCre contains ARSH and CEN6 for maintenance in Saccharomyces cerevisiae. pKlNatCre has a Kluyveromyces lactis replication origin and centromere in addition. Copyright © 2001 John Wiley & Sons, Ltd.