Application of caged fluorescent nucleotides to live-cell RNA imaging
Application of caged fluorescent nucleotides to live-cell RNA imaging
复制标题
笼状荧光核苷酸在活细胞 RNA 成像中的应用
DOI：
10.1007/978-1-62703-535-4_24
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
A.
中科院分区：
文献类型：
--
作者：
Okamoto;A.
A caged fluorescent nucleic acid probe, which contains a nucleotide modified with one photolabile nitrobenzyl unit and two hybridization-sensitive thiazole orange units, has been synthesized for area-specific fluorescence imaging of RNA in a cell. The probe emits very weak fluorescence regardless of the presence of the complementary RNA, whereas it shows hybridization-sensitive fluorescence emission after photoirradiation for uncaging. Such probes are generated via several chemical synthetic steps and are applicable to area-specific RNA imaging in a cell. Only probes that exist in the defined irradiation area are activated via uncaging irradiation.
