The DNA Integrity Number ( DIN ) Provided by the Genomic DNA ScreenTape Assay Allows for Streamlining of NGS on FFPE Tissue Samples Application Note
The DNA Integrity Number ( DIN ) Provided by the Genomic DNA ScreenTape Assay Allows for Streamlining of NGS on FFPE Tissue Samples Application Note
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基因组 DNA ScreenTape 检测提供的 DNA 完整性编号 (DIN) 可简化 FFPE 组织样本上的 NGS 应用说明
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发表时间：
2014
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通讯作者：
S. Song
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作者：
S. Song
FFPE存档组织的基因组DNA（gDNA）测序可能具有挑战性，因为所获得的材料通常具有可变的质量。本研究表明，使用Agilent Genomic DNA ScreenTape检测试剂盒通过gDNA质量控制获得的DNA完整性编号（DIN）可显著节省测序和样品制备开销。在总共751个FFPE样本中，对197个样本的子集进行了测试，以确定各种NGS参数与DIN的相关性。艾德发现DIN与10倍下的靶向率和覆盖率等关键参数之间存在相关性。因此，将QC阈值设定为≥ 3 DIN，从而排除了65%（n = 488）的总样本集，并节省了大量时间和精力。
Sequencing of genomic DNA (gDNA) from FFPE archived tissue can be challenging, as the obtained material is often of variable quality. This study demonstrates that the DNA Integrity Number (DIN) obtained by the quality control of gDNA using the Agilent Genomic DNA ScreenTape assay has allowed for a pronounced saving of sequencing and sample preparation overhead. Out of a total of 751 FFPE samples, a subset of 197 were tested for a correlation of various NGS parameters against the DIN. A correlation was identifi ed between DIN and the key parameters of on-target rate and coverage at 10x. A QC threshold of ≥ 3 DIN was therefore set, which consequently excluded 65 % (n = 488) of the total sample set and saved a signifi cant amount of time and effort.