PRESENCE OF TI (WT31) NEGATIVE LYMPHOCYTES-T IN NORMAL BLOOD AND THYMUS
PRESENCE OF TI (WT31) NEGATIVE LYMPHOCYTES-T IN NORMAL BLOOD AND THYMUS
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DOI：
10.1038/324268a0
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发表时间：
1986-11-20
期刊：
影响因子：
64.8
通讯作者：
WEISS, A
中科院分区：
文献类型：
--
作者：
LANIER, LL;WEISS, A
大多数T淋巴细胞上表达的抗原受体是由α链和β链亚单位1-6组成的二硫键连接的异源二聚体(Ti)。在人类T淋巴细胞表面，Ti与三种不变蛋白非共价相关，分别是CD3-γ，-δ和-ε7。已有研究表明，Ti是表达CD3的必要条件。但最近从胎儿血液中建立的T白血病细胞系10、依赖IL-2(IL-2)的T细胞克隆以及免疫缺陷伴裸淋巴细胞综合征和外胚叶发育不良综合征患者建立的IL-2依赖细胞系12已被证明表达CD3，而不是Ti13(由于单抗WT31而检测到)。这些淋巴细胞可能表达T细胞抗原受体γ(TcR-γ)基因的产物，而不是与CD310，12相关的α/β异二聚体。初步研究表明，在正常淋巴组织10，12中，表达−但缺乏钛的T细胞频率很低。胸腺中CD3+、WT31−细胞的频率很低(占胸腺细胞总数的0.2-0.9%)，这表明这一群体并不代表胸腺发育的一个主要阶段，可能是一个独特的T细胞谱系。
The antigen receptor expressed on most T lymphocytes is a disulphide-linked heterodimer (Ti) that is composed ofα-chain andβ-chain subunits1–6. On the surface of human T lymphocytes, Ti is non-covalently associated with three invariant proteins, designated CD3-γ, -δ, and -ε7. It has been suggested that Ti is obligatory for CD3 expression8,9. But a T leukaemia cell line10, IL-2 (interleukin 2) dependent T-cell clones established from fetal blood11and IL-2 dependent cell lines established from immunodeficiency patients with bare lymphocyte syndrome and ectodermal dysplasia syndrome12have recently been shown to express CD3, but not Ti13(detected due to monoclonal antibody WT31). These lymphocytes may express the product of the T-cell antigen receptorγ(TCR-γ) gene, rather than theα/βheterodimer, in association with CD310,12. Preliminary studies suggested that T cells expressing CD3 but lacking Ti are present in low frequency in normal lymphoid tissues10,12. Here we show that in normal blood and thymus CD3+, WT31−T cells express neither CD4 nor CD8. The low frequency (<0.2–0.9% of total thymocytes) of CD3+, WT31−cells in the thymus suggests that this population does not represent a major stage of thymic development and may be a distinct lineage of T cells.