Angiotensin II activates mitogen-activated protein kinase via protein kinase C and Ras/Raf-1 kinase in bovine adrenal glomerulosa cells.
Angiotensin II activates mitogen-activated protein kinase via protein kinase C and Ras/Raf-1 kinase in bovine adrenal glomerulosa cells.
复制标题
血管紧张素 II 在牛肾上腺肾小球细胞中通过蛋白激酶 C 和 Ras/Raf-1 激酶激活丝裂原激活蛋白激酶。
DOI：
10.1210/endo.139.4.5865
复制
发表时间：
1998
期刊：
影响因子：
4.8
通讯作者：
K. Catt
中科院分区：
文献类型：
--
作者：
Y. Tian;R. Smith;T. Balla;K. Catt
血管紧张素II（Ang II）刺激牛肾上腺肾小球细胞的生长和有丝分裂，但很少有人知道介导这些反应的信号通路。对培养的牛肾上腺肾小球细胞中的生长促进途径的分析显示，Ang II通过AT 1受体起作用，引起丝裂原活化蛋白激酶（MAPK）的快速但短暂的激活，ED 50为10-50 pM。虽然无论是Ca 2+内流或Ca 2+释放从细胞内的商店是不够的激活MAPK，Ca 2+似乎在这种反应中发挥了许可的作用。血管紧张素II诱导的MAPK激活的一个主要组成部分是不敏感的百日咳毒素（PTX），虽然一个较小的PTX敏感的组件不能被排除在外。Ang Ⅱ还诱导ras和raf-1激酶的快速激活，其时间过程与MAPK的时间过程相关。蛋白激酶C（PKC）的激活佛波醇12-肉豆蔻酸酯13-乙酸酯是足够的激活MAPK和raf-1激酶。然而，尽管PKC耗竭对Ang II诱导的raf-1激酶激活没有影响，但它减弱了Ang II诱导的MAPK激活。血管紧张素II还刺激了迁移率的raf-1，反映了过度磷酸化的激酶。然而，与其激活不同，raf-1过度磷酸化依赖于PKC，其时间过程与激活无关，而是与激酶的失活有关。总之，这些研究结果表明，血管紧张素II刺激多种途径，MAPK激活通过PKC和ras/raf-1激酶在牛肾上腺肾小球细胞。
Angiotensin II (Ang II) stimulates growth and mitogenesis in bovine adrenal glomerulosa cells, but little is known about the signaling pathways that mediate these responses. An analysis of the growth-promoting pathways in cultured bovine adrenal glomerulosa cells revealed that Ang II, acting via the AT1 receptor, caused rapid but transient activation of mitogen-activated protein kinase (MAPK), with an ED50 of 10-50 pM. Although neither Ca2+ influx nor Ca2+ release from intracellular stores was sufficient to activate MAPK, Ca2+ appeared to play a permissive role in this response. A major component of Ang II-induced MAPK activation was insensitive to pertussis toxin (PTX), although a minor PTX-sensitive component could not be excluded. Ang II also induced the rapid activation of ras and raf-1 kinase with time-courses that correlated with that of MAPK. Activation of protein kinase C (PKC) by phorbol 12-myristate 13-acetate was sufficient to activate both MAPK and raf-1 kinase. However, whereas PKC depletion had no effect on Ang II-induced raf-1 kinase activation, it attenuated Ang II-induced MAPK activation. Ang II also stimulated a mobility shift of raf-1, reflecting hyperphosphorylation of the kinase. However, unlike its activation, raf-1 hyperphosphorylation was dependent on PKC and its time-course correlated not with activation, but rather with deactivation of the kinase. Taken together, these findings indicate that Ang II stimulates multiple pathways to MAPK activation via PKC and ras/raf-1 kinase in bovine adrenal glomerulosa cells.
登录
查看更多内容
影响因子：
8.3
作者：
BERK, BC;VEKSHTEIN, V;TSUDA, T
通讯作者：
TSUDA, T
Ras 和 Raf 参与 Gi 偶联乙酰胆碱毒蕈碱 m2 受体激活丝裂原激活蛋白 (MAP) 激酶激酶和 MAP 激酶。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Winitz,S;Russell,M;Qian,NX;Gardner,A;Dwyer,L;Johnson,GL
通讯作者：
Johnson,GL
血管紧张素 II 诱导新生大鼠心脏成纤维细胞中蛋白酪氨酸磷酸化。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Schorb,W;Peeler,TC;Madigan,NN;Conrad,KM;Baker,KM
通讯作者：
Baker,KM
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cao,Y;Skurat,AV;DePaoli-Roach,AA;Roach,PJ
通讯作者：
Roach,PJ
影响因子：
4.4
作者：
Chu, Van Trung;Enghard, Philipp;Berek, Claudia
通讯作者：
Berek, Claudia