The Correlation Between the Internal Structure and Vascularization of Controllable Porous Bioceramic Materials In Vivo: A Quantitative Study
The Correlation Between the Internal Structure and Vascularization of Controllable Porous Bioceramic Materials In Vivo: A Quantitative Study
复制标题
体内可控多孔生物陶瓷材料内部结构与血管化的相关性：定量研究
DOI：
10.1089/ten.tea.2010.0148
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发表时间：
2010-12-01
影响因子：
4.1
通讯作者：
Huang, Xin
中科院分区：
文献类型：
--
作者：
Bai, Feng;Wang, Zhen;Huang, Xin
值得注意的是，生物材料的多孔结构特征在支架的血管化中起着重要作用。然而，由于未能准确控制生物材料的多孔结构，因此关于血管化的最佳条件，包括大孔尺寸、形状、互连和大孔排列，尚未达成共识。为了研究多孔结构参数对生物材料血管化的影响，需要准确控制这些参数。本研究以组装有机微球为模板，结合流延成型技术制备了孔参数精确可控的多孔β-磷酸三钙（β-TCP）。使用这种技术，我们生产了一系列的圆盘型β-TCP与可变的孔径和可变的互连，以评估大孔尺寸和互连的生物陶瓷材料在体内的血管化的影响。通过组织形态学和单光子发射计算机断层扫描评价植入兔模型中的β-TCP的血管化。结果表明，孔隙参数不仅影响血管生长到多孔结构的大小，而且还影响血管形成的数量的生物陶瓷的孔隙。孔径的增加仅导致生长到生物陶瓷支架的大孔中的血管的尺寸增加。然而，随着互连尺寸的增加，在大孔中形成的血管的尺寸和数量都增加。因此，我们得出结论，与孔径相比，互连的尺寸对于支架中的血管化更重要。另一方面，在孔径大于400 μ m的支架中，血管化没有显著差异，并且随着孔径大于400 μ m的进一步增加，血管化程度没有显著增加，表明血管化的孔径上限为400 μ m。
It is noticeable that porous architectural characteristics of the biomaterials play an important role in revascularization of the scaffold. However, there has been no consensus regarding the optimal conditions for vascularization, including macropore size, shape, interconnection, and the arrangement of macropores, due to the failure to accurately control porous structure of biomaterials. To investigate the effect of the porous structure parameters on vascularization of the biomaterials, an accurate control of these parameters is required. In this study, porous beta-tricalcium phosphate (beta-TCP) with accurately controlled pore parameters is fabricated by using assembled organic microspheres as templates combined with casting technique. Using this technique, we produced a series of disk-type beta-TCP with variable pore sizes and variable interconnections to evaluate the influence of macropore size and interconnection on the vascularization of bioceramic material in vivo. The vascularization of beta-TCP implanted in the rabbit model is evaluated by histomorphology and single photon emission computed tomography. The results showed that the pore parameters affect not only the size of the blood vessels growing into the porous structure but also the number of blood vessels formed in the pores of the bioceramic. The increase in pore size only resulted in an increase in size of the blood vessels growing into the macroporous of the bioceramic scaffolds. However, with the increase in size of interconnection, both the size and number of the blood vessels formed in the macroporous increased. Therefore, we conclude that the size of the interconnections is more important for vascularization in the scaffold compared with the pore size. On the other hand, there was no significant difference in vascularization in the scaffolds with pores size above 400 mu m, and there was no marked increase in extent of vascularization with further increase in pore size above 400 mu m, indicating that the upper limit of pore size for vascularization is 400 mu m.