Enhanced Diffusion of Catalytically Active Enzymes
Enhanced Diffusion of Catalytically Active Enzymes
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DOI：
10.1021/acscentsci.9b00228
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发表时间：
2019-05
影响因子：
18.2
通讯作者：
Yifei Zhang;H. Hess
中科院分区：
文献类型：
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作者：
Yifei Zhang;H. Hess
在过去的十年中，已经看到了越来越多的研究，催化活性酶的增强扩散。这些研究表明，当酶催化反应或与配体结合时，它们被主动推动（例如，底物或抑制剂）。在本展望中，我们按时间顺序总结和讨论了实验观察和理论解释，并强调了这些努力中的潜在矛盾。我们指出，现有的多聚体形式的酶或同工酶可能会导致文物的测量和底物结合后的构象变化通常不足以引起扩散增强大于30%。因此，迫切需要更严格的实验和更全面的理论来定量验证和描述酶扩散增强。
The past decade has seen an increasing number of investigations into enhanced diffusion of catalytically active enzymes. These studies suggested that enzymes are actively propelled as they catalyze reactions or bind with ligands (e.g., substrates or inhibitors). In this Outlook, we chronologically summarize and discuss the experimental observations and theoretical interpretations and emphasize the potential contradictions in these efforts. We point out that the existing multimeric forms of enzymes or isozymes may cause artifacts in measurements and that the conformational changes upon substrate binding are usually not sufficient to give rise to a diffusion enhancement greater than 30%. Therefore, more rigorous experiments and a more comprehensive theory are urgently needed to quantitatively validate and describe the enhanced enzyme diffusion.