Multimodal fluorescence-mediated tomography and SPECT/CT for small-animal imaging.
Multimodal fluorescence-mediated tomography and SPECT/CT for small-animal imaging.
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DOI：
10.2967/jnumed.112.105742
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发表时间：
2013-04
期刊：
影响因子：
--
通讯作者：
Culver JP
中科院分区：
文献类型：
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作者：
Solomon M;Nothdruft RE;Akers W;Edwards WB;Liang K;Xu B;Suddlow GP;Deghani H;Tai YC;Eggebrecht AT;Achilefu S;Culver JP
核图像和光学图像的空间和时间联合配准将使来自这些互补的分子成像模式的信息能够融合。集成中的一个关键挑战是将光学硬件安装到核成像平台中。基于柔性纤维的荧光介导层析成像(FMT)系统提供了一种可行的解决方案，因为小动物核成像系统的各种成像孔径大小可以潜在地容纳FMT光纤成像阵列。此外，FMT成像有助于及时联合配准核对比度和光学对比度。在此，我们将基于光纤的FMT系统与临床前NanoSPECT/CT平台相结合。通过跟踪单分子多模式显像剂(MOMIA)在大鼠前哨淋巴结(SLN)中的积聚，证明了体内成像的可行性。基于光纤的视频率FMT成像系统由12个交替源(785 nm和830 nm激光二极管)和13个探测器组成。为了保持高时间采样，系统在每个探测器处同时采集比率度量数据。使用归一化Born方法重建数据，其中三维有限元模型来自大鼠的解剖CT图像，用于精确的光传播建模。使用MOMIA集成了核对比度和光学对比度。在将MOMIA皮内注射到前爪后，立即开始数据收集，同时使用SPECT和CT获取FMT数据。来自MOMIA的荧光和放射性被共同定位在空间上重合的区域。活体成像与手术暴露的淋巴结证实了光学对比剂的定位。通过将SPECT作为DOT重建的先验，将光学和核对比度结合在一起。建立了基于光纤的视频速率FMT系统与商业临床前NanoSPECT/CT平台集成的可行性。MOMIA在共同配准的FMT-SPECT-CT图像上的空间重合区域的共同定位可能会促进下一代临床前和临床多模式光学-核平台的开发，用于广泛的成像应用。由于SPECT、CT和FMT成像提供了互补的信息，这种方法有可能阐明与癌症诊断和治疗监测相关的潜在生物学过程。
Spatial and temporal co-registration of nuclear and optical images would enable the fusion of the information from theses complementary molecular imaging modalities. A critical challenge in integration is fitting optical hardware into the nuclear imaging platforms. Flexible fiber-based fluorescence mediated tomography (FMT) systems provide a viable solution because the various imaging bore sizes of small animal nuclear imaging systems can potentially accommodate the FMT fiber imaging arrays. Further, FMT imaging facilitates co-registering the nuclear and optical contrasts in time. Herein, we combine a fiber based FMT system with a preclinical NanoSPECT/CT platform. Feasibility of in vivo imaging is demonstrated by tracking the accumulation of a monomolecular multimodal imaging agent (MOMIA) in a sentinel lymph node (SLN) of a rat. The fiber-based, video-rate FMT imaging system is composed of 12 alternating sources (785nm and 830nm LDs) and 13 detectors. To maintain high temporal sampling, the system simultaneously acquires ratio-metric data at each detector. The data is reconstructed using the normalized Born approach with a three-dimensional finite element model derived from an anatomical CT image of a rat for accurate light propagation modeling. Nuclear and optical contrasts are integrated by using a MOMIA. Data collection begins immediately after injection of the MOMIA intradermally into the forepaw with the FMT data acquired simultaneously with both the SPECT and CT. Fluorescence and radioactivity from the MOMIA were co-localized in a spatially coincident region. Intravital imaging with surgical exposure of the lymph node validated the localization of the optical contrast. The optical and nuclear contrasts where integrated by incorporating SPECT as a prior in the DOT reconstruction. The feasibility of integrating a fiber-based, video-rate FMT system with a commercial preclinical NanoSPECT/CT platform was established. The co-localization of the MOMIA in a spatially coincident region on the co-registered FMT-SPECT-CT image may facilitate the development of the next generation preclinical and clinical multimodal optical-nuclear platform for a broad array of imaging applications. Due to the complementary information provided by the SPECT, CT and FMT imaging, this approach has potential to elucidate the underlying biological processes relevant to cancer diagnosis and therapy monitoring.