Cytotoxicity of phenolic acid phenethyl esters on oral cancer cells
Cytotoxicity of phenolic acid phenethyl esters on oral cancer cells
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DOI：
10.1016/j.canlet.2004.09.048
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发表时间：
2005-06-01
期刊：
影响因子：
9.7
通讯作者：
Ho, LK
中科院分区：
文献类型：
--
作者：
Lee, YT;Don, MJ;Ho, LK
酚酸苯乙酯类化合物具有多种生物活性，包括抗癌活性。本文合成了14个衍生物，并对其体外细胞毒活性进行了评价。用MTT比色法和台盼蓝拒染法分别检测了这些衍生物对口腔鳞状细胞癌（SAS）、口腔表皮样癌孟1（OEC-M1）和正常人口腔成纤维细胞（NHOF）生长的抑制作用。咖啡酸苯乙酯，3a（CAPE），3b，3c和3d显示对SAS和OEC-M1细胞系的细胞毒性作用，但在5-100 μ M剂量范围内对NHOF细胞系没有。流式细胞仪分析显示3c可使OEC-M1细胞阻滞于G（2）/M期。这种对代表性癌症和正常细胞的差异作用表明这些化合物可能在口腔癌化疗中有用。(c)2004爱思唯尔爱尔兰有限公司保留所有权利。
Many phenolic acid phenethyl esters possess diverse biological effects including anti-cancer activity. A series of 14 derivatives were synthesized for the evaluation of their cytotoxic effect on oral cancer cells. These derivatives were tested by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric and trypan blue dye exclusion assay on the growth of oral squamous cell carcinoma (SAS), oral epidermoid carcinoma-Meng 1 (OEC-M1), and normal human oral fibroblast (NHOF) cells, respectively. Caffeic acid phenethyl esters, 3a (CAPE), and 3b, 3c, and 3d showed cytotoxic effects on the SAS and OEC-M1 cell lines, but not the NHOF cell line at a 5-100 mu M dose range. Flow cytometric analysis showed that 3c caused OEC-M1 cell arrest at G(2)/M phase. Such differential effects on representative cancer and normal cells suggested these compounds might be useful in oral cancer chemotherapy. (c) 2004 Elsevier Ireland Ltd. All rights reserved.