Oxygen tension influences DNA fragmentation and cell death in glucocorticoid-treated thymocytes.
Oxygen tension influences DNA fragmentation and cell death in glucocorticoid-treated thymocytes.
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氧张力影响糖皮质激素处理的胸腺细胞中的 DNA 断裂和细胞死亡。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
C. Caldarera
中科院分区：
文献类型：
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作者：
C. Stefanelli;I. Stanic’;F. Bonavita;C. Muscari;C. Pignatti;C. Rossoni;C. Caldarera
在氧气迅速还原至0.5%以下的95%N_2/5%CO_2气氛中，可抑制地塞米松诱导的大鼠胸腺细胞核小体间DNA断裂和细胞死亡。在高氧(95%O2)培养的细胞中，DNA断裂在5%氧气中较轻的缺氧条件下延迟，而细胞死亡增加。另一方面，长时间的缺氧会导致自发性细胞死亡的增加。低氧也能抑制钙离子载体A23187诱导的DNA断裂，但不能抑制拓扑异构酶抑制剂喜树碱引起的DNA断裂。这些数据支持氧反应物种参与钙介导的细胞凋亡的假说，并表明氧在细胞程序性死亡的调节中起着复杂的作用。
Internucleosomal DNA fragmentation and cell death induced by dexamethasone in rat thymocytes were inhibited when cells were cultured in 95% N2/5% CO2 atmosphere, in which oxygen was rapidly reduced to under 0.5%. DNA fragmentation was delayed by a less severe hypoxia in 5% oxygen whilst in cell cultured in high oxygen atmosphere (95% O2) cell death was increased. On the other hand, prolonged oxygen deprivation caused an increase of spontaneous apoptotic cell death. Hypoxia also inhibited DNA fragmentation induced by calcium ionophore A23187, but not by topoisomerase inhibitor camptothecin. These data support the hypothesis of the involvement of oxygen reactive species in calcium-mediated apoptosis and suggest a complex role of oxygen in the modulation of programmed cell death.