Heme oxygenase (HO)-1 expressing macrophages/microglial cells accumulate during oligodendroglioma progression
Heme oxygenase (HO)-1 expressing macrophages/microglial cells accumulate during oligodendroglioma progression
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DOI：
10.1016/s0006-8993(00)02594-4
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发表时间：
2000-11-03
期刊：
影响因子：
2.9
通讯作者：
Schluesener, HJ
中科院分区：
文献类型：
--
作者：
Deininger, MH;Meyermann, R;Schluesener, HJ
血红素加氧酶（HO-1，HSP 32）催化血红素氧化为胆绿素和一氧化碳，一种假定的神经递质。在脑中，HO-1表达与氧化应激和缺氧期间的神经保护相关。然而，连续的下游介导参与新血管生成和随后的肿瘤生长。我们分析了HO-1表达在69少突胶质细胞瘤组织样本，在大鼠颅内移植的C6胶质瘤，和神经病理学未改变的控制大脑免疫组化。双标记实验证实了HO-1表达细胞的性质。逆转录聚合酶链反应用于证明HO-1基因表达。HO-1免疫反应主要见于巨噬细胞/小胶质细胞。原发性少突胶质细胞瘤中HO-1表达的巨噬细胞/小胶质细胞数量显著低于其配对的复发性少突胶质细胞瘤（P
Heme oxygenase (HO-1, HSP32) catalyzes the oxidation of heme to biliverdin and carbon monoxide, a putative neurotransmitter. In the brain, HO-1 expression has been associated with neuroprotection during oxidative stress and hypoxia. However, consecutive downstream mediation is involved in neoangiogenesis and consequent neoplastic outgrowth. We have analyzed HO-1 expression in 69 oligodendroglioma tissue samples, in rat intracranially transplanted C6 gliomas, and neuropathologically unaltered control brains by immunohistochemistry. Double labeling experiments confirmed the nature of HO-1 expressing cells. Reverse transcription-polymerase chain reaction was used to demonstrate HO-1 gene expression. HO-1 immunoreactivity was predominantly observed in macrophages/ microglial cells. The number of HO-1 expressing macrophages/microglial cells was significantly lower in primary oligodendrogliomas than in their matched relapses (P