Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis
Mass mortality of cultured sevenband grouper, Epinephelus septemfasciatus, associated with viral nervous necrosis
复制标题
DOI：
10.3147/jsfp.31.165
复制
发表时间：
1996-09-01
期刊：
影响因子：
0.6
通讯作者：
Nakai, T
中科院分区：
文献类型：
--
作者：
Fukuda, Y;Nguyen, HD;Nakai, T
调查了大分县养殖的七带石斑鱼Epinealus septemfasciatus的群体死亡率。1993年和1994年的夏季（7月至10月;水温25-28 ℃）发生了该病的暴发。受影响的鱼体重在170至1，850克之间，其特征是倒置游泳和鱼鳔膨胀。组织学观察到中枢神经组织和视网膜的坏死和空泡化。虽然从受影响的鱼中未检测到与疾病相关的细菌或寄生虫，并且在RTG-2，BF-2和EPC细胞系上未观察到CPE，但在大多数受影响的鱼的受影响神经细胞的细胞质中发现了大量直径为28 nm的球形病毒颗粒。根据抗SJNNV兔血清的荧光抗体技术试验和SJNNV外壳蛋白基因扩增引物的聚合酶链反应试验结果，该病毒被鉴定为鱼类诺达病毒（神经坏死病毒）。这些结果表明，七带石斑鱼的死亡至少部分是由于病毒性神经坏死（VNN）。这是首次报道七带石斑鱼的VNN，也是成鱼的首例VNN。
Mass mortalities of sevenband grouper Epinephelus septemfasciatus reared in Oita Prefecture were investigated. The outbreaks of the disease occurred in summer seasons (July to October; water temperature 25-28 degrees C) of 1993 and 1994. Affected fish ranging 170 to 1,850g in body weight were characterized by upside down swimming and inflation of swimbladder. Histopathologically, necrosis and vacuolation in the central nervous tissues and retina were observed. Although bacteria or parasites associated with the disease were not detected from affected fish and no CPE was observed on RTG-2, BF-2, and EPC cell lines, spherical virus particles, 28 nm in diameter, were found abundantly in the cytoplasm of affected nerve cells of most of the affected fish. The virus was identified as a fish nodavirus (nervous necrosis virus), based on the results of fluorescent antibody technique test with anti-SJNNV rabbit serum and polymerase chain reaction test with primers for amplification of SJNNV coat protein gene. These results suggest that the mortalities of sevenband grouper were at least partly due to viral nervous necrosis (VNN). This is the first report describing VNN of sevenband grouper and the first case of VNN in the adult fish.