Induction of nicotine in tobacco by herbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars
Induction of nicotine in tobacco by herbivory and its relation to glucose oxidase activity in the labial gland of three noctuid caterpillars
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发表时间：
2004
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通讯作者：
ZONGNa;WANGChenzhu
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作者：
ZONGNa;WANGChenzhu
烟草是棉铃虫Helicoverpa armigera（Huibner）、烟夜蛾Helicoverpa assulta Guenee和斜纹夜蛾Spodoptera litura（Fabricius）的寄主植物。叶片烟碱响应的差异，这三个幼虫的喂养和机械模拟他们的喂养进行了检查，通过HPLC。结果表明，H. armigera和H.烟青虫幼虫取食或用其唇腺提取物处理模拟损伤。在机械损伤叶区处理时，尼日尔曲霉的葡萄糖氧化酶也抑制了烟碱的产生。相反，S.斜纹夜蛾幼虫取食或用其唇腺提取物模拟损伤处理。热变性不能抵消S.斜纹夜蛾唇腺提取物对烟草烟碱产生的影响。用酶法测定了两种黑腹果蝇唇腺提取液中葡萄糖氧化酶的活性。armigera和H. assulta，但H.棉铃虫对棉铃虫的抗性显著高于棉铃虫。assulta.未检测到唇腺提取物中葡萄糖氧化酶活性。斜纹。表明毛虫唇腺葡萄糖氧化酶活性在烟碱对取食的反应中起重要作用。葡萄糖氧化酶主要存在于唇腺中。在pH 7.0时活性最高。D-葡萄糖是葡萄糖氧化酶的最适底物。唇腺葡萄糖氧化酶活性在幼虫发育过程中每天变化，当幼虫积极进食时发现高活性。
Tobacco Nicotiana tabacum L. is a host plant ofHelicoverpa armigera (Huibner), Helicoverpa assulta Guenee and Spodoptera litura (Fabricius) (Lepidoptera, Noctuidae). The difference in leaf nicotine response to the feeding by these three larvae and the mechanical simulation of their feeding was examined by HPLC. Results indicated that nicotine induction was suppressed by H. armigera and H. assulta larvae feeding or by simulated damage treated with their labial glands extracts. The production of nicotine was also suppressed by the glucose oxidase from Aspergillus niger when it was treated on mechanically wounded leaf area. On the contrary, the nicotine production was stimulated by S. litura larva feeding or by simulated damage treated with its labial gland extract. Heat denature can not counteract the stimulation effect of the S. litura labial gland extracts to tobacco nicotine production. The glucose oxidase activity was detected in labial gland extracts of both H. armigera and H. assulta, but the activity in H. armigera was significantly higher than that in H. assulta. No glucose oxidase activity was detected in labial gland extracts of S. litura. It is shown that the glucose oxidase activity in labial glands of caterpillars plays an important role in the nicotine response to herbivory. The glucose oxidase was mainly contained in the labial gland of H. armigera larva, and had the highest activity at pH 7.0. D-Glucose was the optimal substrate of the glucose oxidase. Labial gland glucose oxidase activities varied daily during larval development with high activities found when larvae were actively feeding.