Bone marrow stroma-derived prolactin is involved in basal and platelet-activating factor-stimulated in vitro erythropoiesis.
Bone marrow stroma-derived prolactin is involved in basal and platelet-activating factor-stimulated in vitro erythropoiesis.
复制标题
骨髓基质衍生的催乳素参与基础和血小板激活因子刺激的体外红细胞生成。
作者：
G. Bellone;P. Astarita;E. Artusio;S. Silvestri;Katia Mareschi;A. Turletti;S. Buttiglieri;G. Emanuelli;L. Matera
在体外外源性催乳素（PRL），粒细胞-巨噬细胞集落刺激因子（GM-CSF），白细胞介素-3（IL-3）在体外红系分化的早期步骤之间的合作已在以前的研究中显示。为了更深入地了解PRL在体内造血中的作用，我们现在已经讨论了内源性PRL在骨髓（BM）基质环境中造血祖细胞生长中的参与。局部PRL生产的炎症介质血小板活化因子（PAF），这是已知的BM细胞和调节垂体PRL释放产生的可能调制，也进行了评估。在存在抗人PRL抗体的情况下，来自在BM基质细胞（BMSC）层上培养的CD 34+造血祖细胞的爆发形成单位红系（BFU-E）集落的发育轻微但显著地减少。PAF预处理BMSC可增加共培养CD 34+细胞的BFU-E集落形成率，而这种作用可被PAF抗血清完全阻断。通过酶联免疫斑点（Elispot）技术证实PAF调节BMSC释放PRL。此外，免疫沉淀和蛋白质印迹实验表明，在BMSC培养基中的两个免疫反应产物。这些对应于非糖基化（23 kD）和糖基化（25.5 kD）形式的垂体PRL，也表达的B-淋巴母细胞系IM 9-P3。PAF处理后，非糖基化形式的特异性增加和糖基化形式的减少。聚合酶链反应（PCR）扩增的逆转录RNA使用PRL特异性引物显示PRL的信息在BMSC和IM 9-P3细胞的存在。与人PRL mRNA交叉反应的大鼠PRL cDNA探针的原位杂交实验证实其存在于一小部分未刺激的BMSC和大多数PAF刺激的BMSC中。PAF对PRL介导的集落形成、PRL释放和mRNA激活的增强作用可通过用PAF受体（R）拮抗剂WEB 2170预处理BMSC来抵消。最后，BMSC对PAF的反应性通过这些细胞上的PAF-R mRNA的存在来证实。
Cooperation between in vitro exogenous prolactin (PRL), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interleukin-3 (IL-3) at an early step of in vitro erythroid differentiation has been shown in a previous study. To gain more insight into the role of PRL in in vivo hematopoiesis, we have now addressed the involvement of endogenous PRL in the growth of hematopoietic progenitors in a bone marrow (BM) stroma environment. The possible modulation of local PRL production by the inflammatory mediator platelet-activating factor (PAF), which is known to be produced by BM cells and to regulate pituitary PRL release, has also been evaluated. Development of burst-forming unit-erythroid (BFU-E) colonies from CD34+ hematopoietic progenitors cultured on a BM stroma cells (BMSC) layer was slightly, but significantly, reduced in the presence of an anti-human PRL antibody. Pretreatment of BMSC with PAF increased the BFU-E colony efficiency of cocultured CD34+ cells, and this effect was completely abrogated by the antiserum. PAF-modulated release of PRL by BMSC was confirmed by an enzyme-linked-immunospot (Elispot) technique. In addition, immunoprecipitation and Western blotting experiments showed two immunoreactive products in the BMSC culture medium. These corresponded to the nonglycosylated (23 kD) and glycosylated (25.5 kD) forms of pituitary PRL that are also expressed by the B-lymphoblastoid cell line IM9-P3. Specific increase of the nonglycosylated form and decrease of the glycosylated form was observed after PAF treatment. Polymerase chain reaction (PCR) amplification of reverse transcribed RNA using PRL-specific primers showed the presence of PRL message in BMSC and IM9-P3 cells. In situ hybridization experiments with a rat PRL cDNA probe cross-reacting with human PRL mRNA confirmed its presence in a small fraction of unstimulated BMSC and in the majority of PAF-stimulated BMSC. The enhancing effect of PAF on PRL-mediated colony formation, PRL release, and mRNA activation was counteracted by pretreating BMSC with the PAF-receptor (R) antagonist WEB 2170. Lastly, responsiveness of BMSC to PAF was substantiated by the presence of the PAF-R mRNA on these cells.
编码血小板激活因子功能受体的人类 cDNA 的表征。
DOI：
10.1016/s0006-291x(05)81261-6
发表时间：
1991
影响因子：
3.1
作者：
Ye,RD;Prossnitz,ER;Zou,AH;Cochrane,CG
通讯作者：
Cochrane,CG
影响因子：
1.3
作者：
E. Deryugina;C. Müller-Sieburg
通讯作者：
E. Deryugina;C. Müller-Sieburg
NK细胞刺激因子/IL-12对人体造血的双重刺激和抑制作用。
DOI：
--
发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Bellone,G;Trinchieri,G
通讯作者：
Trinchieri,G