The local environment determines the assembly of root endophytic fungi at a continental scale
The local environment determines the assembly of root endophytic fungi at a continental scale
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DOI：
10.1111/1462-2920.13112
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发表时间：
2016-08-01
影响因子：
5.1
通讯作者：
Macia-Vicente, Jose G.
中科院分区：
文献类型：
--
作者：
Glynou, Kyriaki;Ali, Tahir;Macia-Vicente, Jose G.
根内生真菌存在于多种植物和生态系统中，但其地理分布的生态驱动因素知之甚少。在这里，我们调查的发生在非菌根植物属Microthlaspi的根内生菌，和环境因素和地理距离的影响，在大陆尺度上构建自己的社区。我们在北方地中海和中欧的52个植物种群中取样，并使用栽培方法研究它们的内生群落。根培养产生了2601个菌株，通过对1998个代表性菌落进行内部转录间隔区测序，将其分为296个操作分类单位（OTU）。气候和空间因子是内生植物群落结构的最佳描述因子，其重要性超过了土壤特性、寄主基因型和地理距离。OTU丰富度的负影响降水量，并遵循降水和温度的纬度梯度的群落组成。只有6个广泛分布的OTU属于Pleosporales，Hypocreales和Helotiales的订单代表约50%的所有分离物。对它们的个体分布的评估揭示了特定的生态偏好或世界性的发生。我们的研究结果支持了当地环境的强烈影响，在确定根内生菌群落，并显示了不同的生态位占用个别内生菌。
Root endophytic fungi are found in a great variety of plants and ecosystems, but the ecological drivers of their biogeographic distribution are poorly understood. Here, we investigate the occurrence of root endophytes in the non-mycorrhizal plant genus Microthlaspi, and the effect of environmental factors and geographic distance in structuring their communities at a continental scale. We sampled 52 plant populations across the northern Mediterranean and central Europe and used a cultivation approach to study their endophytic communities. Cultivation of roots yielded 2601 isolates, which were grouped into 296 operational taxonomic units (OTUs) by internal transcribed spacer sequencing of 1998 representative colonies. Climatic and spatial factors were the best descriptors of the structure of endophytic communities, outweighing soil characteristics, host genotype and geographical distance. OTU richness was negatively affected by precipitation, and the composition of communities followed latitudinal gradients of precipitation and temperature. Only six widespread OTUs belonging to the orders Pleosporales, Hypocreales and Helotiales represented about 50% of all isolates. Assessments of their individual distribution revealed particular ecological preferences or a cosmopolitan occurrence. Our findings support a strong influence of the local environment in determining root endophytic communities, and show a different niche occupancy by individual endophytes.