Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.
Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination.
复制标题
家蚕 P 元素体细胞抑制剂 (BmPSI) 是家蚕雄性性别决定的关键辅助因素
DOI：
10.1371/journal.pgen.1006576
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发表时间：
2017-01
期刊：
影响因子：
4.5
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Xu J;Chen S;Zeng B;James AA;Tan A;Huang Y
昆虫性别决定途径的操纵为有利于农业和公共卫生的广泛战略提供了基础。此外，昆虫在导致性别分化的遗传途径中表现出显着的多样性。家蚕作为一种益虫已经被人类养殖了两千多年，最近又被作为研究鳞翅目遗传学和发育的模型系统。以前的研究已经确定了B。mori Fem皮尔纳作为主要的雌性决定因子，BmMasc作为其下游靶标，而雄性性别决定的遗传情况仍不清楚。在本研究中，我们利用转基因CRISPR/Cas9系统在基因BmSxl、Bmtra 2、BmImp、BmImpM、BmPSI和BmMasc中产生一组全面的敲除突变，以研究它们在家蚕性别决定中的作用。Bmtra 2的缺失导致Bmdsx转录物的完全耗尽，这是性别决定途径中保守的下游因子，并诱导胚胎致死。BmImp或BmImPM功能的丧失不影响性分化。BmPSI和BmMasc基因的突变影响Bmdsx的剪接和雌性生殖器出现在雄性外生殖器中。有趣的是，我们确定BmPSI调节BmMasc，BmImpM和Bmdsx的表达，支持的结论，它作为一个关键的辅助因素在家蚕雄性性别决定。
Manipulation of sex determination pathways in insects provides the basis for a wide spectrum of strategies to benefit agriculture and public health. Furthermore, insects display a remarkable diversity in the genetic pathways that lead to sex differentiation. The silkworm, Bombyx mori, has been cultivated by humans as a beneficial insect for over two millennia, and more recently as a model system for studying lepidopteran genetics and development. Previous studies have identified the B. mori Fem piRNA as the primary female determining factor and BmMasc as its downstream target, while the genetic scenario for male sex determination was still unclear. In the current study, we exploite the transgenic CRISPR/Cas9 system to generate a comprehensive set of knockout mutations in genes BmSxl, Bmtra2, BmImp, BmImpM, BmPSI and BmMasc, to investigate their roles in silkworm sex determination. Absence of Bmtra2 results in the complete depletion of Bmdsx transcripts, which is the conserved downstream factor in the sex determination pathway, and induces embryonic lethality. Loss of BmImp or BmImpM function does not affect the sexual differentiation. Mutations in BmPSI and BmMasc genes affect the splicing of Bmdsx and the female reproductive apparatus appeared in the male external genital. Intriguingly, we identify that BmPSI regulates expression of BmMasc, BmImpM and Bmdsx, supporting the conclusion that it acts as a key auxiliary factor in silkworm male sex determination.