Lutein transport systems loaded with rice protein-based self-assembled nanoparticles
Lutein transport systems loaded with rice protein-based self-assembled nanoparticles
复制标题
装载有大米蛋白自组装纳米粒子的叶黄素运输系统
DOI：
10.1016/j.fbio.2021.101061
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发表时间：
2021-04-24
期刊：
影响因子：
5.2
通讯作者：
Zhu, Xue-Mei
中科院分区：
文献类型：
--
作者：
Ma, Xiao-Yu;Chen, Xian-Xin;Zhu, Xue-Mei
以大米蛋白酶解物为载体，构建了叶黄素（Lut）的微囊化纳米粒，提高了叶黄素的溶解度。采用抗溶剂法研究了三种不同水解度（DH）（低（2%）、中（4%）和高（8%））的大米蛋白（RP），RPH蛋白浓度（1、2、5和10 mg/mL）和Lut水平（0.25-1.5 mg/mL）对制备Lut-RPH的影响。通过粒径、zeta电位、载量、包封率和形态学等表征Lut-RPH。并对Lut与RPH的相互作用机理进行了初步探讨。使用具有三个DH的RPH包封的Lut纳米颗粒的EE分别为94.1、89.8和90.5%，而LC分别为23.5、22.4和22.6%。Lut与RPH之间的相互作用表明，RPH的荧光逐渐被Lut以剂量依赖的方式淬灭。Lut-RPH的结合常数K-a和结合位点数均高于Lut-RP，说明RPH与Lut-RP之间存在较强的结合力。这些实验证实了该绿色、安全的Lut-RPH工艺的成功，为将来Lut-RPH在营养强化食品、营养补充剂和药物传递中的应用提供了重要信息。
The solubility of lutein (Lut) was improved through the construction of encapsulated nanoparticles using rice protein hydrolysates (RPH) as nanocarriers. The effects of rice protein (RP) with three different degrees of hydrolysis (DH) including low (2%), medium (4%) and high (8%), with RPH protein concentrations (1, 2, 5 and 10 mg/mL) and Lut levels (0.25-1.5 mg/mL) on the preparation of Lut-RPH were studied using an antisolvent method. Particle size, zeta-potential, loading content (LC), encapsulation efficiency (EE) and morphology were analysed to characterize Lut-RPH. The interaction mechanism between Lut and RPH was also investigated. The EE of Lut nanoparticles encapsulated using RPH with three DH were 94.1, 89.8 and 90.5%, respectively, while LC was 23.5, 22.4 and 22.6%, respectively. The interaction between Lut and RPH suggested that the RPH fluorescence was gradually quenched by the addition of Lut in a dose-dependent manner. The higher binding constants K-a and more binding sites of Lut-RPH than that of Lut-RP suggested there was a strong binding force between RPH and Lut. These experiments confirmed the sucess of the green and safe Lut-RPH process, which will provide important information for the future application of Lut in nutritional fortified foods, supplements and drug delivery.