BMP2-loaded hollow hydroxyapatite microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects.
BMP2-loaded hollow hydroxyapatite microspheres exhibit enhanced osteoinduction and osteogenicity in large bone defects.
复制标题
负载 BMP2 的中空羟基磷灰石微球在大骨缺损中表现出增强的骨诱导和成骨性。
DOI：
10.2147/ijn.s74677
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发表时间：
2015
影响因子：
8
通讯作者：
Tang Z
中科院分区：
文献类型：
--
作者：
Xiong L;Zeng J;Yao A;Tu Q;Li J;Yan L;Tang Z
大面积骨缺损的再生是一个骨诱导、骨传导和成骨过程，通常需要骨移植物支持。与天然自体或同种异体骨移植物相关的局限性表明需要合成替代物。本研究探讨了使用新型中空羟基磷灰石微球作为骨传导基质和骨形态发生蛋白2（BMP 2），一种有效的骨再生诱导剂的控制性局部递送载体的可行性。采用玻璃转化法制备了中空羟基磷灰石微球（100±25 μm），核为60±18 μm，壳为介孔结构（180±42 m2/g表面积），并负载重组人BMP 2（1 μg/mg）。在最初的48小时内，BMP 2从微球温和地爆发释放到周围的磷酸盐缓冲盐水中，并以低速率持续超过40天。与不含BMP 2的空心羟基磷灰石微球或不含载体的可溶性BMP 2相比，负载BMP 2的空心羟基磷灰石微球具有显著增强的重建兔桡骨骨缺损的能力，如增加血清碱性磷酸酶;在12周内快速且完全的新骨形成;以及较大的生物力学弯曲强度。这些结果表明，BMP-2负载的空心羟基磷灰石微球可能是一个潜在的新的选择，骨移植替代物在骨再生。
The regeneration of large bone defects is an osteoinductive, osteoconductive, and osteogenic process that often requires a bone graft for support. Limitations associated with naturally autogenic or allogenic bone grafts have demonstrated the need for synthetic substitutes. The present study investigates the feasibility of using novel hollow hydroxyapatite microspheres as an osteoconductive matrix and a carrier for controlled local delivery of bone morphogenetic protein 2 (BMP2), a potent osteogenic inducer of bone regeneration. Hollow hydroxyapatite microspheres (100±25 μm) with a core (60±18 μm) and a mesoporous shell (180±42 m2/g surface area) were prepared by a glass conversion technique and loaded with recombinant human BMP2 (1 μg/mg). There was a gentle burst release of BMP2 from microspheres into the surrounding phosphate-buffered saline in vitro within the initial 48 hours, and continued at a low rate for over 40 days. In comparison with hollow hydroxyapatite microspheres without BMP2 or soluble BMP2 without a carrier, BMP2-loaded hollow hydroxyapatite microspheres had a significantly enhanced capacity to reconstitute radial bone defects in rabbit, as shown by increased serum alkaline phosphatase; quick and complete new bone formation within 12 weeks; and great biomechanical flexural strength. These results indicate that BMP2-loaded hollow hydroxyapatite microspheres could be a potential new option for bone graft substitutes in bone regeneration.