Effects of nonylphenol and triclosan on production of plasma vitellogenin and testosterone in male South African clawed frogs (Xenopus laevis)
Effects of nonylphenol and triclosan on production of plasma vitellogenin and testosterone in male South African clawed frogs (Xenopus laevis)
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DOI：
10.1248/bpb.28.1748
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发表时间：
2005-09-01
影响因子：
2
通讯作者：
Arizono, K
中科院分区：
文献类型：
--
作者：
Matsumura, N;Ishibashi, H;Arizono, K
研究了壬基酚（NP）和三氯生（TCS）对雄性南非爪蛙（Xenopus laevis）卵黄原蛋白（Vg）、睾酮(T)和肝细胞色素P450 1A和2B活性的影响。在14 d的水接触试验中，对照、10、50、100 μ g/l NP和20、100、200 μ g/l TCS处理组雄性蛙血浆Vg合成水平无显著差异。2、20、200 μ g/g体重NP雄性蛙腹腔注射后，对照组和各处理组血浆Vg水平无显著差异。然而，所有TCS处理组（腹腔注射4、40和400 μ g/g体重组）血浆Vg水平均低于溶剂对照组，且高剂量NP或TCS雄性青蛙的T水平低于对照组。肝细胞色素P450 1A和2B活性在各处理组间无显著差异。注射20 μ g/g体重雌二醇-17 β后，雄蛙血浆Vg水平显著高于对照组。这些结果表明，雄性非洲爪蟾血浆Vg和T的产生谱可以作为检测激素活性物质的有用生物标志物。
We investigated the effects of nonylphenol (NP) and triclosan (TCS) on production of vitellogenin (Vg), testosterone (T), and hepatic cytochrome P450 1A and 2B activities in male South African clawed frogs (Xenopus laevis). In a 14-d waterborne exposure test, no significant differences in the level of plasma Vg synthesis in male frogs were observed among the control, 10, 50, and 100 mu g/l NP and 20, 100, and 200 mu g/l TCS treatment groups. Intraperitoneal injection of male frogs with 2, 20, and 200 mu g/g body weight NP resulted in no significant differences in plasma Vg levels among the control and all treatment groups. However, the levels of plasma Vg in all TCS treatment, groups (intraperitoneal injection of 4, 40, and 400 mu g/g body weight) were lower than that in the solvent control group, and male frogs injected with high doses of NP or TCS bad lower T levels than the control group. No significant differences in hepatic cytochrome P450 1A and 2B activities were observed among the all treatment groups. Male frogs injected with 20 mu g/g body weight of estradiol-17 beta had significantly higher plasma Vg levels than the control group. These results suggest that profiles of plasma Vg and T production in male Xenopus laevis could be useful biomarkers for detecting hormonally active agents.