Characterization of the stimulator cells in the murine primary mixed leukocyte response.
Characterization of the stimulator cells in the murine primary mixed leukocyte response.
复制标题
小鼠初级混合白细胞反应中刺激细胞的表征。
作者：
M. Minami;D. Shreffler;C. Cowing
检查巨噬细胞富集和耗竭的脾细胞群刺激小鼠1度混合白细胞应答的能力。未分级脾细胞、Sephadex g-10通过的脾细胞、4小时玻璃贴壁和24小时玻璃贴壁脾细胞的定量比较显示，1级MLR刺激细胞具有吞噬作用，T和B淋巴细胞不能刺激应答。通过使用几个INB;艾德和重组小鼠品系与MHC的不同亚区同源，发现巨噬细胞富集的细胞能够诱导针对K、D、I-A和I-E差异以及针对K和D突变体的MLR。当刺激物和应答物在I-J、I-C、S或G处不同时，巨噬细胞富集和耗尽的脾细胞均不能刺激应答。此外，由K或D区差异单独诱导的MLR刺激依赖于表达Ia抗原的刺激细胞。因此，似乎只有少数表达相关MHC同种抗原的细胞亚群在鼠1度MLR中具有免疫原性。
Macrophage-enriched and depleted spleen cell populations were examined for their ability to stimulate a murine 1 degree mixed leukocyte response. A quantitative comparison of unfractionated spleen cells, Sephadex g-10 passed spleen cells, 4-hr glass adherent and 24-hr glass adherent spleen cells revealed that a potent 1 degree MLR stimulator cell was phagocytic and that T and B lymphocytes failed to stimulate a response. By using several inb;ed and recombinant mouse strains congenic for different subregions of the MHC, it was found that macrophage-enriched cells were capable of inducing an MLR against K, D, I-A, and I-E differences and against K and D mutants. Both macrophage-enriched and depleted spleen cells failed to stimulate responses when the stimulator and responder differed at I-J, I-C, S, or G. Moreover, MLR stimulation induced by K or D region differences alone was dependent upon stimulator cells that expressed Ia antigens. Therefore, it would appear that only a minor subpopulation of the cells that express the relevant MHC alloantigens is immunogenic in the murine 1 degree MLR.