Assessment of loci for DNA barcoding in the genus Thrips (Thysanoptera:Thripidae)
Assessment of loci for DNA barcoding in the genus Thrips (Thysanoptera:Thripidae)
复制标题
DOI：
10.1111/j.1755-0998.2009.02723.x
复制
发表时间：
2010-01-01
影响因子：
7.7
通讯作者：
Boonham, N.
中科院分区：
文献类型：
--
作者：
Glover, R. H.;Collins, D. W.;Boonham, N.
仅通过形态来鉴定具有重要经济意义的进口蓟马物种的幼体可能具有挑战性，通常需要进行培养。对于欧盟检疫物种，如棕榈蓟马，快速、准确的识别至关重要。使用细胞色素氧化酶 I (COI) 基因的 DNA 条形码已成为物种鉴定的流行技术；然而，在一些无脊椎动物属中，COI 已被证明不能为物种区分提供足够的变异性。本研究对五个不同基因座进行了比较，以研究它们区分少数蓟马物种的能力。所有五个位点都通过邻接树来区分物种，并且根据对种内和种间距离的进一步调查确定了不同程度的歧视。在 T. Palmi 中观察到两个不同的 COI 进化枝，并在考虑来自其他四个额外基因座的数据和地理收集数据时判断为 COI 单倍型。 COI 被证明可以提供足够的变异，可用于未来蓟马属 DNA 条形码工作。
Identification of the juveniles of economically important thrips species on imports by morphology alone can be challenging and culturing is usually required. In the case of EU quarantine species such as Thrips palmi, rapid and accurate identification is essential. DNA barcoding using the Cytochrome oxidase I (COI) gene has become a popular technique for species identification; however, in some invertebrate genera COI has been shown to provide insufficient variability for species discrimination. This study presents a comparison of five different loci to investigate their ability to discriminate a small number of Thrips species. All five loci discriminated the species by neighbour-joining tree and varying degrees of discrimination were determined upon further investigation of the intraspecific and interspecific distances. Two distinct COI clades were observed for T. Palmi and judged to be COI haplotypes when data from the other four additional loci and geographical collection data were taken into consideration. COI was shown to provide sufficient variation to be used in future DNA barcoding efforts within the genus Thrips.