CHARACTERIZATION OF MONOCLONAL-ANTIBODIES PROTECTING MICE AGAINST RICKETTSIA-RICKETTSII
CHARACTERIZATION OF MONOCLONAL-ANTIBODIES PROTECTING MICE AGAINST RICKETTSIA-RICKETTSII
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DOI：
10.1093/infdis/151.6.1052
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发表时间：
1985-01-01
影响因子：
6.4
通讯作者：
THOMAS, LA
中科院分区：
文献类型：
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作者：
ANACKER, RL;LIST, RH;THOMAS, LA
杂交瘤产生的单克隆抗体的R。为研究立克次体抗原的功能，从小鼠体内制备了立克次体抗原。在迄今为止检测的31个反应性杂交瘤系的IG亚类中，11个属于IgG 2A亚类，9个属于IgG 2B亚类，7个属于IgG 3亚类; 4个不与任何同种型血清反应。五种抗体识别分子上存在的表位，这些分子被认为是多糖和MW异质性。20个单克隆抗体与170，000-道尔顿抗原反应，6个沉淀133，000和32，000-道尔顿多肽。只有那些针对170，000和133，000道尔顿抗原的抗体才能保护小鼠免受R.立克次氏体在落基山斑疹热康复期患者的血清中检测到这些相同抗原的抗体。所有的单克隆抗体反应的抗原显然位于立克次体表面。这些抗体的保护活性与它们在补体结合、酶联免疫吸附试验和免疫荧光试验中的反应性无关。
Hybridomas producing monoclonal antibodies to R. rickettsii were prepared from mice to investigate the function of rickettsial antigens. Of the 31 reactive hybridoma lines thus far tested for Ig subclasses, 11 belonged to the IgG2A subclass, 9 to the IgG2B subclass and 7 to the IgG3 subclass; 4 did not react with any of the isotyping sera. Five of the antibodies recognized epitopes present on molecules that were presumed to be polysaccharide and heterogeneous in MW. Twenty monoclonal antibodies reacted with a 170,000-dalton antigen and 6 precipitated the 133,000 and 32,000-dalton polypeptides. Only those antibodies to the 170,000- and 133,000-dalton antigens protected mice from challenge with R. rickettsii. Antibodies to these same antigens were detected in sera from patients convalescing from Rocky Mountain spotted fever. All monoclonal antibodies reacted with antigens apparently located on the rickettsial surface. The protective activity of these antibodies was not correlated with their reactivity in complement fixation, enzyme-linked immunosorbent assay and immunofluorescence tests.