Fabrication and characterization of six electrospun poly(α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications
Fabrication and characterization of six electrospun poly(α-hydroxy ester)-based fibrous scaffolds for tissue engineering applications
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DOI：
10.1016/j.actbio.2006.02.005
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发表时间：
2006-07-01
影响因子：
9.7
通讯作者：
Tuan, Rocky S.
中科院分区：
文献类型：
--
作者：
Li, Wan-Ju;Cooper, James A., Jr.;Tuan, Rocky S.
正在研究用于组织工程应用的最常见的合成生物可降解聚合物是FDA批准的临床使用的聚（α-羟基酯）。为了更好地评估用于支架制造的电纺技术的适用性，六种常用的聚（α-羟基酯）被用来制备电纺纤维支架，并且它们的物理和生物学性能也被表征。我们的研究结果表明，具体的，优化的制造参数是需要每种聚合物产生的支架，由均匀的结构形态相似的天然细胞外基质。扫描电子显微镜（SEM）揭示了一个高度多孔的，三维结构的所有支架，平均纤维直径范围从300 nm至1.5 μ m，这取决于所使用的聚合物类型。聚（乙醇酸）（PGA）和聚（D，L-乳酸-共-乙醇酸50：50）（PLGA 5050）纤维结构的机械刚度最大，而聚（L-乳酸）（PLLA）和聚（F-己内酯）（PCL）支架的顺应性最好。在生理溶液中孵育后，在PGA、聚（D，L-乳酸）（PDLLA）、PLGA 5050和聚（D，L-乳酸-共-乙醇酸85：15）（PLGA 8515）纤维支架中发现由于聚合物降解导致的严重结构破坏，而PLLA和PCL纤维支架在相同时间段内保持稳健的支架结构，基于油宏观和SEM观察。此外，PLLA支架比其他聚合物支架支持种子细胞（软骨细胞和间充质干细胞）的最高增殖速率。我们的研究结果表明，PLLA和PCL为基础的纤维支架表现出最佳的结构完整性和支持理想的细胞反应在文化，这表明这种支架可能是有前途的候选生物材料的组织工程应用。(c)2006 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The most common synthetic biodegradable polymers being investigated for tissue engineering applications are FDA approved, clinically used poly(alpha-hydroxy esters). To better assess the applicability of the clectrospinning technology for scaffold fabrication, six commonly used poly(alpha-hydroxy esters) were used to prepare clectrospun fibrous scaffolds, and their physical and biological properties were also characterized. Our results suggest that specific, optimized fabrication parameters are required for each polymer to produce scaffolds that consist of uniform structures morphologically similar to native extracellular matrix. Scanning electron microscopy (SEM) revealed a highly porous, three-dimensional structure for all scaffolds, with average fiber diameter ranging from 300 nm to 1.5 mu m, depending on the polymer type used. The poly(glycolic acid) (PGA) and poly(D,L-lactic-co-glycolic acid 50:50) (PLGA5050) fibrous structures were mechanically stiffest, whereas the poly(L-lactic acid) (PLLA) and poly(F-caprolactone) (PCL) scaffolds were most compliant. Upon incubation in physiological solution, severe structural destruction due to polymer degradation was found in the PGA, poly(D,L-lactic acid) (PDLLA), PLGA5050, and poly(D,L-lactic-co-glycolic acid 85:15) (PLGA8515) fibrous scaffolds, whereas PLLA and PCL fibrous scaffolds maintained a robust scaffold structure during the same time period, based oil macroscopic and SEM observations. In addition, PLLA scaffolds supported the highest rate of proliferation of seeded cells (chondrocytes and mesenchymal stem cells) than other polymeric scaffolds. Our findings showed that PLLA and PCL based fibrous scaffolds exhibited the most optimal structural integrity and supported desirable cellular response in culture, suggesting that such scaffolds may be promising candidate biomaterials for tissue engineering applications. (c) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.