Antibacterial screening of Rumex species native to the Carpathian Basin and bioactivity-guided isolation of compounds from Rumex aquaticus
Antibacterial screening of Rumex species native to the Carpathian Basin and bioactivity-guided isolation of compounds from Rumex aquaticus
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DOI：
10.1016/j.fitote.2017.03.009
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发表时间：
2017-04-01
期刊：
影响因子：
3.4
通讯作者：
Vasas, Andrea
中科院分区：
文献类型：
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作者：
Orban-Gyapai, Orsolya;Liktor-Busa, Erika;Vasas, Andrea
属于酸模属（Polygonaceae）的植物在传统医学中在全世界用于治疗由不同微生物引起的各种疾病（例如与细菌相关的皮肤病、痢疾和肠炎）。本研究的重点是对原产于喀尔巴阡盆地的酸模属植物进行抗菌筛选，并从最有效的物种之一水生酸模中分离化合物。研究了14种酸模不同部位甲醇提取物的正己烷、氯仿和水提物的抑菌作用。acetosella、河（R. acetosa，R.阿尔皮努斯山R. aquaticus、河（R. conglomeratus，R. crispus、河hydrolapathum，河钝叶藓obtusifolius、R.钝叶藓subalpinus、河（R. patientia、R.普赫尔河scutatus、河stenophyllus和R. thyrsiflorus）对表皮葡萄球菌（Staphylococcus epidermidis）、S.金黄色葡萄球菌、MRSA、枯草芽孢杆菌、卡他莫拉菌、化脓链球菌、S.肺炎链球菌（S.无乳杆菌、铜绿假单胞菌、大肠埃希菌和肺炎克雷伯菌。主要是从植物根制备的正己烷和氯仿提取物对一种或多种细菌菌株显示出高的抗菌活性（抑菌圈> 15 mm）。采用高效液相色谱法对川楝子地上部分和根的活性成分进行了分离纯化。水生物进行多步分离程序。19种化合物，其中包括萘（musizin，及其葡萄糖苷，torachryone-glucoside，2-methoxystypandrone），蒽醌类（大黄素、大黄酚、大黄素甲醚、柠檬苦苷、大黄酚-8-O-葡萄糖苷）、黄酮类（槲皮素、槲皮素-3，3 '-二甲醚、异山奈素、槲皮素3-O-阿拉伯糖苷、槲皮素3-O-半乳糖苷、儿茶素）、芪类（白藜芦醇，白藜芦醇苷）和1-硬脂酰甘油。对分离得到的化合物进行了抑菌活性测定，发现萘类化合物对几种细菌有显著的抑菌作用。(C)2017 Elsevier B. V.版权所有。
Plants belonging to the genus Rumex (family Polygonaceae) are used worldwide in traditional medicine for the treatment of various diseases caused by different microorganisms (e.g. bacteria-related dermatologic conditions, dysentery and enteritis). The present study focused on the antibacterial screening of Rumex species native to the Carpathian Basin, and isolation of compounds from one of the most efficient species, Rumex aquaticus. The antibacterial effects of n-hexane, chloroform and aqueous fractions of methanol extracts prepared from different parts of 14 Rumex species (R. acetosella, R. acetosa, R. alpinus,. R. aquaticus, R. conglomeratus, R. crispus, R. hydrolapathum, R. obtusifolius subsp. obtusifolius, R. obtusifolius subsp. subalpinus, R. patientia, R. pukher, R. scutatus, R. stenophyllus and R. thyrsiflorus) were investigated against Staphylococcus epidermidis, S. aureus, MRSA, Bacillus subtilis, Moraxella catarrhalis, Streptococcus pyogenes, S. pneumoniae, S. agalactiae, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae using the disc diffusion method. Mainly the n-hexane and chloroform extracts prepared from the roots of the plants displayed high antibacterial activity (inhibition zones > 15 mm) against one or more bacterial strains. The highly active extracts of the aerial part and root of R. aquaticus were subjected to a multistep separation procedure. 19 Compounds, among them naphthalenes (musizin, and its glucoside, torachrysone-glucoside, 2-methoxystypandrone), anthraquinones (emodin, chrysophanol, physcion, citreorosein, chrysophanol-8-O-glucoside), flavonoids (quercetin, quercetin-3,3'-dimethylether, isokaempferide, quercetin 3-O-arabinoside, quercetin 3-O-galactoside, catechin), stilbenes (resveratrol, piceid), and 1-stearoylglycerol were isolated from the plant. The antibacterial activities of isolated compounds were determined, and it was observed that especially naphthalenes exerted remarkable antibacterial effects against several bacterial strains. (C) 2017 Elsevier B.V. All rights reserved.