A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.
A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.
复制标题
DOI：
10.1093/nar/19.12.3345
复制
发表时间：
1991-06
影响因子：
14.9
通讯作者：
J. Van Ness;S. Kalbfleisch;C. R. Petrie;M. Reed;J. Tabone;N. Vermeulen
中科院分区：
文献类型：
--
作者：
J. Van Ness;S. Kalbfleisch;C. R. Petrie;M. Reed;J. Tabone;N. Vermeulen
描述了一种将数量明确的寡脱氧核糖核苷酸（odn）固定在多功能尼龙支架上的方法。固体支撑，尼龙6/6珠，共价涂有聚（乙烯亚胺），以提供一个反应性间隔臂的附着odn。5'-氨基己基尾odn选择性地使用2,4,6-三氯-1,3,5-三嗪（三聚氰胺）活化，然后通过三嗪部分共价连接到头部。改性尼龙支架具有低水平的非特异性核酸结合，并有效捕获RNA和DNA靶标。
A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.