Fluorometric determination of proteins using the terbium (III)-2-thenoyltrifluoroacetone-sodium dodecyl benzene sulfonate-protein system
Fluorometric determination of proteins using the terbium (III)-2-thenoyltrifluoroacetone-sodium dodecyl benzene sulfonate-protein system
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使用铽 (III)-2-噻吩酰基三氟丙酮-十二烷基苯磺酸钠-蛋白质系统荧光测定蛋白质
DOI：
10.1016/j.jlumin.2005.12.047
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发表时间：
2006-12-01
影响因子：
3.6
通讯作者：
Liu, Shufang
中科院分区：
文献类型：
--
作者：
Jia, Zhen;Yang, Jinghe;Liu, Shufang
研究发现，在pH = 8.00的乙二胺（HMTA）-HCl缓冲溶液中，蛋白质对铽（Ⅲ）（Tb ~（3+））-2-噻吩甲酰三氟丙酮（TTA）-十二烷基苯磺酸钠（SDBS）体系的荧光有增强作用。在此基础上，提出了一种灵敏的蛋白质测定方法。实验表明，在最佳条件下，蛋白质浓度在4.0 × 10（-9）~ 7.5 × 10（-6）g/mL范围内（牛血清白蛋白），在5.0 × 10（-9）~ 1.5 × 10（-5）g/mL范围内（人血清白蛋白），卵清蛋白（EA）为1.0 x 10（-8）-7.5 x 10（-6）g/mL。它们的检出限（SIN = 3）分别为0.5、0.8和2.0 ng/mL。并对相互作用机理进行了研究。(c)2006 Elsevier B. V.保留所有权利。
It is found that in hexamethylene tetramine (HMTA)-HCl buffer of pH = 8.00, proteins can enhance the fluorescence of terbium (III) (Tb3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl benzene sulfonate (SDBS) system. Based on this, a sensitive method for the determination of proteins is proposed. The experiments indicate that under the optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of proteins in the range of 4.0 x 10(-9)-7.5 x 10(-6) g/mL for bovine serum albumin (BSA), 5.0 x 10(-9)-1.5 x 10(-5) g/mL for human serum albumin (HSA), 1.0 x 10(-8)-7.5 x 10(-6) g/mL for egg albumin (EA). Their detection limits (SIN = 3) are 0.5, 0.8 and 2.0 ng/mL, respectively. The interaction mechanism is also studied. (c) 2006 Elsevier B.V. All rights reserved.