On the role of the sterol hydroxyl group in membranes.
On the role of the sterol hydroxyl group in membranes.
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关于甾醇羟基在膜中的作用。
DOI：
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发表时间：
1979
影响因子：
4.8
通讯作者：
K. Bloch
中科院分区：
文献类型：
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作者：
A. K. Lala;T. Buttke;K. Bloch
研究了含有封闭的3-羟基的甾醇衍生物用于维持两种甾醇营养缺陷型的生长的充分性。山羊支原体是一种需要胆固醇的细菌，在补充有胆固醇甲基醚或胆固醇乙酸酯的培养基上生长几乎与在游离胆固醇上一样好。从细菌细胞中回收两种衍生物，未发生变化。类似地，胆固醇基甲基醚或麦角固醇基甲基醚替代胆固醇或麦角固醇作为酵母突变体菌株GL 7的固醇源，菌株GL 7在2，3-氧化角鲨烯-羊毛甾醇环化中有缺陷。在需氧或半厌氧生长期间，酵母细胞将一些胆固醇甲基醚脱甲基以释放胆固醇。然而，在严格的厌氧条件下，生长在胆固醇甲醚上的细胞不产生游离甾醇。这些结果上的假定要求的膜功能的游离甾醇羟基的轴承进行了讨论。甾醇酯化似乎不是必不可少的两个微生物系统。
The adequacy of sterol derivatives containing a blocked 3-hydroxyl group for sustaining the growth of two sterol auxotrophs has been investigated. Mycoplasma capricolum, a cholesterol-requiring bacterium, grows nearly as well on media supplemented with cholesteryl methyl ether or cholesteryl acetate as on free cholesterol. The two derivatives are recovered unchanged from the bacterial cells. Similarly, cholesteryl methyl ether or ergosteryl methyl ether replace cholesterol or ergosterol as sterol sources for a yeast mutant, strain GL7, defective in 2,3-oxidosqualene-lanosterol cyclization. During aerobic or semianaerobic growth, yeast cells demethylate some of the cholesteryl methyl ether to free cholesterol. However, cells growing on cholesterol methyl ether under strict anaerobic conditions do not produce free sterol. The bearing of these results on the postulated requirement of a free sterol hydroxyl group for membrane function is discussed. Sterol esterification does not appear to be essential for the two microbial systems.