Green tea extract intake during lactation modified cardiac macrophage infiltration and AMP-activated protein kinase phosphorylation in weanling rats from undernourished mother during gestation and lactation.
Green tea extract intake during lactation modified cardiac macrophage infiltration and AMP-activated protein kinase phosphorylation in weanling rats from undernourished mother during gestation and lactation.
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DOI：
10.1017/s2040174416000647
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发表时间：
2017-04
影响因子：
1.7
通讯作者：
E. Matsumoto;S. Kataoka;Y. Mukai;M. Sato;S. Sato
中科院分区：
文献类型：
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作者：
E. Matsumoto;S. Kataoka;Y. Mukai;M. Sato;S. Sato
母亲的饮食限制往往与后代的心血管疾病有关。本研究旨在探讨哺乳期摄入绿色茶提取物（GTE）对暴露于母体膳食蛋白质限制的断奶仔猪心脏中巨噬细胞浸润以及腺苷酸（AMP）激活的蛋白激酶（AMPK）和丝氨酸-苏氨酸激酶（Akt）活化的影响。妊娠Wistar大鼠在整个妊娠期间喂食对照（C）或低蛋白饮食（LP）。分娩后，母鼠在哺乳期接受对照或含GTE的对照饲料：妊娠期和哺乳期对照饲料（CC）、妊娠期和哺乳期低蛋白饲料（LPC）、妊娠期低蛋白饲料和哺乳期含0.12% GTE的低蛋白饲料（LPL）以及妊娠期低蛋白饲料和哺乳期含0.24% GTE的低蛋白饲料（LPH）。在第22天处死雌性后代。检测血浆生化指标、巨噬细胞浸润、纤维化程度及AMPK和Akt表达水平。LPH组血浆胰岛素水平高于LPC组。LPC的纤维化面积百分比和巨噬细胞数量均高于CC。相反，LPH中的纤维化面积和巨噬细胞数量比LPC中的小（分别为21%和56%）。LPL和LPH中磷酸化AMPK和Akt的水平均高于LPC。总之，母体蛋白质限制可诱导巨噬细胞浸润和胰岛素水平降低。然而，在哺乳期摄入GTE可以抑制巨噬细胞浸润，并通过上调AMPK和胰岛素信号转导恢复胰岛素分泌功能。
Maternal dietary restriction is often associated with cardiovascular disease in offspring. The aim of this study was to investigate the effect of green tea extract (GTE) intake during lactation on macrophage infiltration, and activation of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and serine-threonine kinase Akt (Akt) in the hearts of weanlings exposed to maternal dietary protein restriction. Pregnant Wistar rats were fed control (C) or low-protein diets (LP) throughout gestation. Following delivery, the dams received a control or a GTE-containing control diet during lactation: control diet during gestation and lactation (CC), low-protein diet during gestation and lactation (LPC), low-protein diet during gestation and 0.12% GTE-containing low-protein diet during lactation (LPL), and low-protein diet during gestation and 0.24% GTE-containing low-protein diet during lactation (LPH). The female offspring were sacrificed at day 22. Biochemical parameters in the plasma, macrophage infiltration, degree of fibrosis and expression levels of AMPK and Akt were examined. The plasma insulin level increased in LPH compared with LPC. Percentage of the fibrotic areas and the number of macrophages in LPC were higher than those in CC. Conversely, the fibrotic areas and the macrophage number in LPH were smaller (21 and 56%, respectively) than those in LPC. The levels of phosphorylated AMPK in LPL and LPH, and Akt in LPH were greater than those in LPC. In conclusion, maternal protein restriction may induce macrophage infiltration and the decrease of insulin levels. However, GTE intake during lactation may suppress macrophage infiltration and restore insulin secretion function via upregulation of AMPK and insulin signaling in weanlings.