Ontogenic and phylogenic studies of intestinal, hepatic, and placental alkaline phosphatases. Evidence that intestinal alkaline phosphatase is a late evolutionary development.
Ontogenic and phylogenic studies of intestinal, hepatic, and placental alkaline phosphatases. Evidence that intestinal alkaline phosphatase is a late evolutionary development.
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肠道、肝脏和胎盘碱性磷酸酶的个体发生和系统发育研究。
DOI：
10.1016/0016-5085(86)90558-5
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发表时间：
1986
期刊：
影响因子：
29.4
通讯作者：
Alpers,DH
中科院分区：
文献类型：
--
作者：
Komoda,T;Koyama,I;Nagata,A;Sakagishi,Y;DeSchryver-Kecskemeti,K;Alpers,DH
我们用生物化学、免疫学和组织学技术研究了豚鼠和大鼠器官磷酸酶的宫内发育。在所有的器官测试的成人磷酸酶活性的功能，实现在第二或第三妊娠期。在大鼠中，组织非特异性磷酸酶活性的免疫沉淀与抗血清的大鼠肝磷酸酶，发现在所有妊娠阶段的肝脏和胎盘。妊娠第一阶段的高肝脏活性对应于造血细胞。肝细胞磷酸酶直到第二妊娠期才出现。组织非特异性磷酸酶活性在第一和第二妊娠期的肠上皮细胞表面，即使在隐窝形成发生。一旦在绒毛上的肠上皮细胞中出现磷酸酶，则仅检测到胰蛋白酶型酶。碱性磷酸酶在肝脏和肠从动物在不同的门测量。鱼类和爬行动物的肠道活动具有组织非特异性酶的酶学特征。肠道碱性磷酸酶的出现具有独特的性质和高比活性是哺乳动物的一个特征。
We have studied the intrauterine development of guinea pig and rat organ phosphatases using biochemical, immunologic, and histologic techniques. In all organs tested the features of the adult phosphatase activity were achieved during the second or third gestational phases. In the rat, the tissue-unspecific phosphatase activity was found by immunoprecipitation with antiserum to rat liver phosphatase in all gestational phases in liver and placenta. The high liver activity in the first phase of gestation corresponded to hematopoietic cells. Hepatocyte phosphatase did not appear until the second gestational phase. The tissue-unspecific phosphatase activity was found in the first and second gestational phase in surface intestinal epithelial cells, even after crypt formation occurred. Once phosphatase appeared in enterocytes on villi, only the intestinal-type enzyme was detected. Alkaline phosphatase was measured in the liver and intestine from animals in various phyla. In fish and reptiles, the intestinal activity had the enzymatic characteristics of the tissue-unspecific enzyme. The appearance of intestinal alkaline phosphatase with unique properties and high specific activity is a characteristic of mammals.