Limiting Pseudomonas aeruginosa Biofilm Formation Using Cold Atmospheric Pressure Plasma
Limiting Pseudomonas aeruginosa Biofilm Formation Using Cold Atmospheric Pressure Plasma
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DOI：
10.1615/plasmamed.2018028325
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发表时间：
2018-09
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影响因子：
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通讯作者：
B. Patenall;H. Hathaway;A. Sedgwick;N. Thet;G. Williams;Amber;E. Young;S. Allinson;R. Short;A. Jenkins
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文献类型：
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作者：
B. Patenall;H. Hathaway;A. Sedgwick;N. Thet;G. Williams;Amber;E. Young;S. Allinson;R. Short;A. Jenkins
我们研究了使用冷大气压（CAP）等离子体破坏和限制铜绿假单胞菌生物膜生长的能力。在生物膜成熟期间的不同时间点评估生物膜暴露于氦气（CAP）射流的效果。结果表明，在生物膜生长期间施加CAP压力的时间量对生物膜在CAP暴露后成熟和恢复的能力具有关键作用。相对于未处理的生物膜，在生物膜形成的早期阶段（0-8小时）期间的干预导致活细菌细胞（在孵育24小时时测量）减少4-5个对数。然而，CAP处理生物膜在12小时及以上仅导致活细胞的2-log减少。这对CAP治疗感染伤口的未来临床应用具有潜在的重要意义。
We investigate the ability to disrupt and limit growth biofilms of Pseudomonas aeruginosa using application of cold atmospheric pressure (CAP) plasma. The effect of the bio-film's exposure to a helium (CAP) jet was assessed at varying time points during biofilm maturation. Results showed that the amount of time during biofilm growth that CAP pressure was applied has a crucial role on the ability of biofilms to mature and recover after CAP exposure. Intervention during the early stages of biofilm formation (0-8 h) results in a 4-5-log reduction in viable bacterial cells (measured at 24 h of incubation) relative to untreated biofilms. However, CAP treatment of biofilm at 12 h and above only results in a 2-log reduction in viable cells. This has potentially important implications for future clinical application of CAP to treat infected wounds.