DNA-sequence rearrangement required for the adaptation of JC polyomavirus to growth in a human neuroblastoma cell line (IMR-32).
DNA-sequence rearrangement required for the adaptation of JC polyomavirus to growth in a human neuroblastoma cell line (IMR-32).
复制标题
JC 多瘤病毒适应人神经母细胞瘤细胞系 (IMR-32) 中生长所需的 DNA 序列重排。
DOI：
10.1006/viro.1993.1659
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发表时间：
1993
期刊：
影响因子：
3.7
通讯作者：
N. Ikegami
中科院分区：
文献类型：
--
作者：
Y. Yogo;K. Hara;J. Guo;F. Taguchi;K. Nagashima;K. Akatani;N. Ikegami
用 JC 多瘤病毒 (JCV) 株 Mad-1 感染人神经母细胞瘤细胞系 (IMR-32)，并随后连续传代，会产生适合在 IMR-32 中生长的病毒（K. Akatani、M. Imai、M. Kimura、K. Nagashima 和 N. Ikegami、J. Med. Virol.，出版中）。为了了解这种适应的基础，我们从适应的病毒中分子克隆了 JCV DNA。克隆的 JCV DNA 基本上由三个物种（M1-IMRa、-IMRb 和 -IMRc）组成，并具有重新排列的调控区域。亲代病毒 Mad-1 的调控区中存在两个 TATA 序列，但在 M1-IMRa、-IMRb 和 -IMRc 中，远离复制起点的一个序列通常被删除。我们证明这些调控区域是 JCV 在 IMR-32 中有效生长所必需的。如果各种 JCV 菌株的调控区域被本研究中定义的区域替换，则应在 IMR-32 中繁殖。由于 JCV 很难在原代人胎儿神经胶质细胞以外的细胞中繁殖，因此该系统可能对 JCV 的结构和免疫学研究有用。
Infection of a human neuroblastoma cell line (IMR-32) with the JC polyomavirus (JCV) strain Mad-1 with subsequent serial passage results in the generation of a virus adapted to growth in IMR-32 (K. Akatani, M. Imai, M. Kimura, K. Nagashima, and N. Ikegami, J. Med. Virol., in press). To understand the basis of this adaptation, we molecularly cloned JCV DNAs from the adapted virus. The cloned JCV DNAs consisted of essentially three species (M1-IMRa, -IMRb, and -IMRc) with rearranged regulatory regions. Two TATA sequences are present in the regulatory region of the parental virus Mad-1, but one distal from the origin of replication was commonly deleted in M1-IMRa, -IMRb, and -IMRc. We showed that these regulatory regions were required for the efficient growth of JCV in IMR-32. Various JCV strains should be propagated in IMR-32, if their regulatory regions are replaced with those defined in this study. Since it is difficult to propagate JCV in cells other than primary human fetal glial cells, this system may be useful for structural and immunological studies of JCV.