Large-scale live imaging of adult neural stem cells in their endogenous niche
Large-scale live imaging of adult neural stem cells in their endogenous niche
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DOI：
10.1242/dev.123018
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发表时间：
2015-10-15
期刊：
影响因子：
4.6
通讯作者：
Bally-Cuif, Laure
中科院分区：
文献类型：
--
作者：
Dray, Nicolas;Bedu, Sebastien;Bally-Cuif, Laure
成体神经干细胞(ANSCs)的活体成像是脊椎动物大脑的一项技术挑战。在这里，我们实现了成年斑马鱼端脑神经源性生态位的长期成像，并通过利用鱼类在近红外波长的透明度和固有的多光子地标，在几周内跟踪了1000个aNSCs的数量。这种方法使我们能够描述aNSCs在整个成体大脑皮层生发区的频率、分布和模式，并突出这些参数的地区差异。
Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.