ILLEGITIMATE TRANSCRIPTION - TRANSCRIPTION OF ANY GENE IN ANY CELL TYPE
ILLEGITIMATE TRANSCRIPTION - TRANSCRIPTION OF ANY GENE IN ANY CELL TYPE
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DOI：
10.1073/pnas.86.8.2617
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发表时间：
1989-04-01
影响因子：
11.1
通讯作者：
KAHN, A
中科院分区：
文献类型：
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作者：
CHELLY, J;CONCORDET, JP;KAHN, A
使用通过聚合酶链式反应进行的cDNA体外扩增，我们检测了各种组织特异性基因（抗苗勒管激素基因，β-珠蛋白、醛缩酶A和因子VIIIc）在人非特异性细胞中的作用，例如成纤维细胞、肝细胞瘤细胞和成淋巴细胞。在大鼠中，红细胞型和肝脏型丙酮酸激酶转录本也在脑，肺和肌肉中检测到。这些“非法”转录本的丰度非常低;然而，它们的存在和通过cDNA聚合酶链反应扩增它们的可能性提供了一个强大的工具，通过使用任何可访问的细胞来分析任何组织特异性基因的病理转录本。
Using in vitro amplification of cDNA by the polymerase chain reaction, we have detected spliced transcripts of various tissue-specific genes (genes for anti-Mullerian hormone, .beta.-globin, aldolase A, and factor VIIIc) in human nonspecific cells, such as fibroblasts, hepatoma cells, and lymphoblasts. In rats, erythroid- and liver-type pyruvate kinase transcripts were also detected in brain, lung, and muscle. The abundance of these "illegitimate" transcripts in very low; yet, their existence and the possibility of amplifying them by the cDNA polymerase chain reaction provide a powerful tool to analyze pathological transcripts of any tissue-specific gene by using any accessible cell.