CRISPR/Cas9-mediated gene-editing technology in fruit quality improvement
CRISPR/Cas9-mediated gene-editing technology in fruit quality improvement
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DOI：
10.1093/fqsafe/fyaa028
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发表时间：
2020-10
影响因子：
5.6
通讯作者：
Xin Xu;Yujin Yuan;Bihong Feng;W. Deng
中科院分区：
文献类型：
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作者：
Xin Xu;Yujin Yuan;Bihong Feng;W. Deng
水果是健康、均衡饮食的重要组成部分，对纤维、必需维生素和微量元素尤为重要。提高水果质量和延长货架期是研究人员的重要目标。然而，传统技术存在周期长、效率低、靶基因修饰困难等缺点，限制了研究的进展。近年来，簇状规则间隔短回文重复序列(CRISPR)/Cas9技术以其高效、简便、低成本等优点发展成为目前最流行的基因编辑技术。CRISPR/Cas9技术被广泛应用于基因功能分析和基因修饰。本文介绍了CRISPR/CAS9技术在水果品质改良中的最新进展。例如，CRISPR/Cas9介导的成熟抑制基因(RIN)、番茄红素去饱和酶(PDS)、果胶裂解酶(PL)、SLMYB12和CLAVATA3(CLV3)的定向突变可以影响果实的成熟、果实生物活性物质、果实质地、果实着色和果实大小。CRISPR/Cas9介导的诱变已成为修饰目的基因和改善果实品质的有效方法。
Fruits are an essential part of a healthy, balanced diet and it is particularly important for fibre, essential vitamins, and trace elements. Improvement in the quality of fruit and elongation of shelf life are crucial goals for researchers. However, traditional techniques have some drawbacks, such as long period, low efficiency, and difficulty in the modification of target genes, which limit the progress of the study. Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 technique was developed and has become the most popular gene-editing technology with high efficiency, simplicity, and low cost. CRISPR/Cas9 technique is widely accepted to analyse gene function and complete genetic modification. This review introduces the latest progress of CRISPR/Cas9 technology in fruit quality improvement. For example, CRISPR/Cas9-mediated targeted mutagenesis of RIPENING INHIBITOR gene (RIN), Lycopene desaturase (PDS), Pectate lyases (PL), SlMYB12, and CLAVATA3 (CLV3) can affect fruit ripening, fruit bioactive compounds, fruit texture, fruit colouration, and fruit size. CRISPR/Cas9-mediated mutagenesis has become an efficient method to modify target genes and improve fruit quality.