INFLUENCE OF ACTIVITY ON MUSCLE SIZE AND PROTEIN TURNOVER
INFLUENCE OF ACTIVITY ON MUSCLE SIZE AND PROTEIN TURNOVER
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DOI：
10.1113/jphysiol.1977.sp011668
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发表时间：
1977-01-01
影响因子：
5.5
通讯作者：
GOLDSPINK, DF
中科院分区：
文献类型：
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作者：
GOLDSPINK, DF
通过将踝关节固定在石膏模型中，研究了[大鼠]肌肉在缩短位置时失去正常活动的影响。由于神经支配在此过程中保持完整，因此移除限制性石膏可以恢复正常活动。比目鱼肌和跖肌生长作为恢复正常活动后的时间的函数，这些组织大小的变化被解释为蛋白质合成和蛋白质分解的平均速率的变化，如通过敏感的体外技术测量的。活动刺激比目鱼肌的蛋白质合成，抑制蛋白质分解，使组织能够积累蛋白质。阻断RNA而不是DNA的从头合成，严重限制了恢复活性后蛋白质合成的正常、快速增强。在延长位置固定后，趾长伸肌的等张活动的恢复未能完全补偿伸展的生长促进影响的损失，并且组织逐渐恢复到对照肌肉的大小。在恢复过程中，较高的蛋白质周转率和RNA浓度的固定肌肉恢复到较低的值的控制。
The effects of the loss of normal activity were studied in [rat] muscles held in a shortened position by immobilizing the ankle in a plaster cast. Since the innervation remained intact with this procedure, removal of the restraining cast allowed restoration of normal activity. The soleus and plantaris muscles grew as a function of time after the return of normal activity and these changes in tissue size were explained by changes in the average rates of protein synthesis and protein break-down as measured by sensitive in vitro techniques. Activity stimulated protein synthesis and inhibited protein break-down of the soleus muscle, enabling the tissue to accumulate protein. Blockage of de novo synthesis of RNA, but not DNA, severely restricted the normal, rapid enhancement of protein synthesis after the return of activity. The return of isotonic activity to the extensor digitorum longus muscle after immobilization in a lengthened position failed to fully compensate for the loss of the growth-promoting influence of stretch and the tissue gradually returned to the size of the control muscle. During the recovery process the higher rate of protein turnover and RNA concentrations of the immobilized muscle returned to the lower values of the control.