Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana
Repression of transcription factor AtWRKY47 confers tolerance to boron toxicity in Arabidopsis thaliana
复制标题
转录因子 AtWRKY47 的抑制表达赋予硼耐受性
DOI：
10.1016/j.ecoenv.2021.112406
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发表时间：
2021-06-10
影响因子：
6.8
通讯作者：
Xu, Fangsen
中科院分区：
文献类型：
--
作者：
Feng, Yingna;Cui, Rui;Xu, Fangsen
Boron (B) excess gives rise to a serious agricultural problem. In this study, we identified a B toxicity responsive transcription factor AtWRKY47 in Arabidopsis thaliana. The T-DNA insertion mutants Atwrky47 showed enhanced tolerance to B toxicity with better growth parameters under high B conditions compared to wild-type Col0 plants. Quantitative analysis of AtWRKY47 mRNA abundance indicated that it was down-regulated under B toxicity conditions. Fluorescently labeled AtWRKY47 protein was localized in nucleus. In contrast to the phenotype of Atwrky47 mutants, overexpression of AtWRKY47 in Col-0 background resulted in lower biomass, less chlorophyll content, and increased sensitivity to B toxicity. More importantly, the B concentration in shoots was higher in the overexpression lines and lower in the Atwrky47 mutants than in Col-0 plants, respectively. These results demonstrate that AtWRKY47 gene plays a key role in regulating plant tolerance to B toxicity.
