EFFECTS OF SOYA BEAN FLAKES AND LICORICE ROOT EXTRACT ON ENZYME-INDUCTION AND TOXICITY IN B6C3F(1) MICE
EFFECTS OF SOYA BEAN FLAKES AND LICORICE ROOT EXTRACT ON ENZYME-INDUCTION AND TOXICITY IN B6C3F(1) MICE
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DOI：
10.1016/0278-6915(93)90189-6
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发表时间：
1993-05-01
影响因子：
4.3
通讯作者：
DABBS, JE
中科院分区：
文献类型：
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作者：
MIRSALIS, JC;HAMILTON, CM;DABBS, JE
此前有报道称，大豆片(SBF)和甘草根提取物(LRE)都具有抗癌特性，这被认为与负责化学致癌物解毒的特定酶的活性增加有关。在雄性B6C3F1小鼠身上进行了为期30天和90天的研究，以确定SBF或LRE诱导的几种解毒酶中的哪一种。饲喂8%和25%LRE的小鼠表现出各种不良的临床体征，体重增加较少，死亡率为30%。SBF组和LRE组的肝脏：体重比均显著增加。在SBF组中没有观察到与治疗相关的重大大体尸检结果。在LRE组中观察到许多异常，包括肾脏、肝脏的病变。脾和胸腺。对90天的肝脏标本进行7-乙氧基香豆素O-脱乙基酶(7-ECOD)、苯并[a]芘羟基酶(BPH)、超氧化物歧化酶(SOD)、谷胱甘肽-S转移酶(GST)和葡萄糖醛酸基转移酶(UDPGT)的分析，并进行30天的中期尸检。没有观察到与治疗相关的BPH或SOD升高。SBF和LRE均可引起UDPGT活性的轻度升高。SBF诱导的GST活性略有升高，而LRE降低了GST活性。7-ECOD活性在LRE组显著升高，SBF组显著降低。在一项为期30天的研究中，LRE和SBF都以不同的剂量水平服用，对样本进行了UDPGT活性检测：与对照组相比，所有剂量组的UDPGT活性都有所下降。结果表明，SBF和LRE均可改变B6C3F1小鼠体内参与化学致癌物解毒的特定酶的活性，但这两种食物在B6C3F1小鼠体内可能不会产生相加效应。
Both soya bean flakes (SBF) and liquorice root extract (LRE) have previously been reported to have anticarcinogenic properties, which have been thought to be related to an increased activity of specific enzymes responsible for the detoxification of chemical carcinogens. 30- and 90-day studies were conducted in male B6C3F1 mice to determine which, if any, of several detoxification enzymes are induced by SBF or LRE. Mice fed 8 and 25% LRE showed a variety of adverse clinical signs, poor weight gain and 30% mortality. Significant increases in liver:body weight ratios were observed in both the SBF and LRE groups. No significant treatment-related gross autopsy findings were observed in any of the SBF groups. A number of abnormalities were observed in the LRE groups, including lesions of the kidney, liver. spleen and thymus. Liver samples from the 90-day study were analysed for 7-ethoxycoumarin O-deethylase (7-ECOD), benzo[a]pyrene hydroxylase (BPH), superoxide dismutase (SOD), glutathione S-transferase (GST) and UDP-glucuronyl transferase (UDPGT) at 90 days, and at an interim 30-day autopsy. No treatment-related increases were observed for BPH or SOD. Both SBF and LRE induced modest increases in UDPGT activity. SBF induced modest increases in GST activity, but LRE decreased this activity. 7-ECOD activity was significantly increased by LRE and decreased by SBF. Samples from a 30-day study in which both LRE and SBF were administered at various dose levels were examined for UDPGT activity: all dose groups showed decreases in UDPGT activity relative to controls. The results suggest that both SBF and LRE may alter the activities of specific enzymes involved in the detoxification of chemical carcinogens: however, the combination of these two foodstuffs may not produce an additive effect in B6C3F1 mice.