FORMATION AND PROPERTIES OF 1000-A-DIAMETER, SINGLE-BILAYER PHOSPHOLIPID VESICLES
FORMATION AND PROPERTIES OF 1000-A-DIAMETER, SINGLE-BILAYER PHOSPHOLIPID VESICLES
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DOI：
10.1073/pnas.76.1.145
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
STRITTMATTER, P
中科院分区：
文献类型：
--
作者：
ENOCH, HG;STRITTMATTER, P
两种方法报道形成大的，均匀大小的磷脂囊泡。这些方法包括处理磷脂，以小的超声囊泡或干燥的脂质膜的形式，脱氧胆酸与磷脂的摩尔比为1:2。随后去除脱氧胆酸盐产生稳定的囊泡制备。这些囊泡以单层为界，平均直径为1000.ang。在Sepharose 4B上通过凝胶过滤很容易从超声囊泡（230 . ang .）中分离出来。从1000年开始。囊泡能够捕获酶和其他大分子，它们可能被证明是有价值的，可以将脂质体包裹的溶质输送到细胞中，也可以定位膜结合蛋白光谱的肽段。
Two methods are reported for the formation of large, uniform-sized phospholipid vesicles. The methods involve the treatment of phospholipid, in the form of either small, sonicated vesicles or a dry lipid film, at a molar ratio of deoxycholate to phospholipid of 1:2. Subsequent removal of deoxycholate yields a stable preparation of vesicles. These vesicles are bounded by a single bilayer, have an average diameter of 1000 .ANG., and are readily separated from sonicated vesicles (230 .ANG.) by gel filtration on Sepharose 4B. Since the 1000-.ANG. vesicles are capable of trapping enzymes and other macromolecules, they may prove valuable for the delivery of liposome-entrapped solutes to cells and for the localization of peptide segments of a spectrum of membrane-bound proteins.