Relationship between matrix proteoglycan content and the effects of salicylate and indomethacin on articular cartilage.
Relationship between matrix proteoglycan content and the effects of salicylate and indomethacin on articular cartilage.
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基质蛋白多糖含量与水杨酸盐和吲哚美辛对关节软骨作用的关系。
DOI：
10.1002/art.1780260412
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发表时间：
1983
影响因子：
--
通讯作者：
Brandt,KD
中科院分区：
文献类型：
--
作者：
Palmoski,MJ;Brandt,KD
当在培养液中加入160 μ g/ml水杨酸钠时，犬股骨髁习惯性负重区的正常关节软骨切片中糖胺聚糖的合成减少了24%（P≤ 0.01）。尽管来自同一关节习惯性卸载区域的软骨中的糖胺聚糖合成与来自负载部位的软骨中的糖胺聚糖合成相似，但前者的平均糖醛酸含量低约25%，并且水杨酸盐对卸载软骨中糖胺聚糖合成的抑制程度要大得多（对照组的42%）比负载软骨中的程度（P≤ 0.01）。此外，1.5 μ g/ml吲哚美辛对负荷区的软骨没有影响，抑制了未负荷区软骨中糖胺聚糖合成的31%。然而，如果用睾丸透明质酸酶处理负荷区域的软骨，吲哚美辛抑制糖胺聚糖合成，并且水杨酸盐对透明质酸酶处理软骨中糖胺聚糖代谢的抑制作用大于未处理软骨。这些数据表明，非甾体类抗炎药对关节软骨中糖胺聚糖代谢的影响依赖于细胞外基质中的蛋白聚糖含量。
When 160 γg/ml sodium salicylate was added to the culture medium, glycosaminoglycan synthesis in slices of normal articular cartilage from habitually loaded areas of canine femoral condyles was diminished by 24% (P≤ 0.01). Although glycosaminoglycan synthesis in cartilage from habitually unloaded regions of the same joints was similar to that in cartilage from loaded sites, the mean uronic acid content of the former was about 25% less, and salicylate suppressed glycosaminoglycan synthesis in unloaded cartilage to a much greater extent (42% of control) than it did in loaded cartilage (P≤ 0.01). Furthermore, 1.5 γg/ml indomethacin, which had no effect on cartilage from loaded regions, suppressed glycosaminoglycan synthesis in cartilage from unloaded regions by 31%. However, if cartilage from loaded regions was treated with testicular hyaluronidase, indomethacin inhibited glycosaminoglycan synthesis, and inhibition of glycosaminoglycan metabolism by salicylate in hyaluronidase‐treated cartilage was greater than in untreated cartilage. The data suggest that the effects of nonsteroidal antiinflammatory drugs on glycosaminoglycan metabolism in articular cartilage are dependent on proteoglycan content of the extracellular matrix.