Involvements of cyclic nucleotide systems in enlarged mice lungs produced by butylated hydroxytoluene.
Involvements of cyclic nucleotide systems in enlarged mice lungs produced by butylated hydroxytoluene.
复制标题
丁基羟基甲苯产生的扩大的小鼠肺中环核苷酸系统的参与。
DOI：
10.1016/0006-2952(78)90177-6
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发表时间：
1978
影响因子：
5.8
通讯作者：
D. Helfman
中科院分区：
文献类型：
--
作者：
J. Kuo;N. Brackett;J. W. Stubbs;M. Shoji;D. Helfman
以400 mg/kg体重的剂量向小鼠单次腹膜内注射丁基化羟基甲苯（BHT），导致其肺部增大，发现这与环核苷酸系统某些参数的某些变化有关。其中包括：（a）增加的环GMP水平，而环AMP水平保持不受影响，（B）增加的环GMP和环AMP的磷酸二酯酶水平，在组织质量增加开始时观察到这些活性的最低比率，（c）增加的环GMP依赖性和环AMP依赖性蛋白激酶水平，和（d）增加的环GMP依赖性酶的刺激性调节剂水平，环腺苷酸依赖性酶的抑制性调节剂水平不变。由于环GMP、环GMP靶酶及其刺激调节剂以及DNA聚合酶活性水平的增加发生在肺肥大和增生之前或与之同时，因此表明环GMP介导的过程可能对BHT致肺毒性损伤后的细胞增殖特别重要。
A single intraperitoneal injection of butylated hydroxytoluene (BHT), at a dose of 400 mg/kg of body weight, to mice caused enlargement of their lungs, which was found to be associated with some modifications in certain parameters of the cyclic nucleotide systems. These include: (a) increased levels of cyclic GMP, with cyclic AMP levels remaining unaffected, (b) increased levels of phosphodiesterases for cyclic GMP and cyclic AMP, with the lowest ratio of these activities seen at the beginning of the tissue mass increase, (c) increased levels of both cyclic GMP-dependent and cyclic AMP-dependent protein kinases, and (d) increased levels of stimulatory modulator of the cyclic GMP-dependent enzyme, with unaltered levels of inhibitory modulator of the cyclic AMP-dependent enzyme. Since increases in levels of cyclic GMP, the cyclic GMP target enzyme and its stimulatory modulator, as well as of the activity of DNA polymerase, occurred prior to or coincided with the pulmonary hypertrophy and hyperplasia, it is suggested that the cyclic GMP-mediated processes may be particularly crucial for cell proliferation after toxic injury of the lung by BHT.