Induction of interleukin 2 receptor beta chain expression by self-recognition in the thymus.
Induction of interleukin 2 receptor beta chain expression by self-recognition in the thymus.
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通过胸腺中的自识别诱导白介素2受体β链表达。
DOI：
10.1084/jem.180.5.1629
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发表时间：
1994-11-01
影响因子：
15.3
通讯作者：
Huenig, Thomas
中科院分区：
文献类型：
--
作者：
Hanke, Thomas;Mitnacht, Rita;Boyd, Richard;Huenig, Thomas
1-2%的成年小鼠胸腺细胞表达T细胞受体α/β（TCR-α/β）以及白细胞介素（IL）2 R β（p70），但不表达α（p55）链。我们表明，先前描述的α/β-TCR + CD 4 -8-和部分重叠的Ly 6C+胸腺细胞包含在这个子集。大多数IL-2 R β + α/β-TCR+细胞具有成熟和活化（热稳定抗原[HSA]-、胸腺共享抗原1 [TSA-1]-、CD 44高、CD 69+）表型。V β 8.2在CD 4 -8-和CD 4和/或CD 8 + IL-2 R β +胸腺细胞中的过度表达表明IL-2 R β表达由TCR介导的活化事件诱导。在H-2Kb特异性TCR的转基因小鼠中，在主流阴性选择条件下IL-2 R β +细胞丰富，即，在Kb的存在下，但在主流正选择或非选择环境的条件下缺席。总之，这些结果表明，除了克隆缺失，未成熟胸腺细胞的自我识别导致一小部分表达IL-2 R β的胸腺细胞表型成熟。IL-2缺陷小鼠含有正常数量的IL-2 R β + α/β-TCR+胸腺细胞，表明与主流T细胞发育一样，这种次要的阳性选择途径不依赖于IL-2。然而，在不存在IL-2的情况下，IL-2 R β +胸腺细胞的CD 4/CD 8亚群组成偏向于CD 4 -8+，主要以CD 4 -8-为代价。一个可能的相关性，这一发现的IL-2缺陷小鼠的免疫病理学的发展进行了讨论。
1-2% of adult mouse thymocytes express the T cell receptor alpha/beta (TCR-alpha/beta) together with the interleukin (IL) 2R beta (p70), but not the alpha (p 55) chain. We show that the previously described alpha/beta-TCR +CD4-8- and the partially overlapping Ly6C+ thymocytes are contained within this subset. Most IL-2R beta+ alpha/beta-TCR+ cells have a mature and activated (heat stable antigen [HSA]-, thymic shared antigen 1 [TSA-1]-, CD44high, CD69+) phenotype. Overrepresentation of V beta 8.2 in both CD4-8- and CD4 and/or CD8+ IL- 2R beta+ thymocytes suggests that IL-2R beta expression is induced by a TCR-mediated activation event. In mice transgenic for an H-2Kb-specific TCR, IL-2R beta+ cells were abundant under conditions of mainstream negative selection, i.e., in the presence of Kb, but absent under conditions of mainstream positive selection or in a nonselecting environment. Together, these results show that in addition to clonal deletion, self-recognition by immature thymocytes leads to phenotypic maturation of a small subset of thymocytes expressing IL-2R beta. IL-2- deficient mice contain normal numbers of IL-2R beta+ alpha/beta-TCR+ thymocytes, indicating that like mainstream T cell development, this minor pathway of positive selection does not depend on IL-2. However, in the absence of IL-2, the CD4/CD8 subset composition of IL-2R beta+ thymocytes is skewed towards CD4-8+, mostly at the expense of CD4-8-. A possible relevance of this finding for the development of the immune pathology of IL-2-deficient mice is discussed.
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影响因子：
15.3
作者：
HAYAKAWA, K;LIN, BT;HARDY, RR
通讯作者：
HARDY, RR
DOI：
10.1073/pnas.89.12.5336
发表时间：
1992-06-15
影响因子：
11.1
作者：
ROCHA, B;VONBOEHMER, H;GUYGRAND, D
通讯作者：
GUYGRAND, D
影响因子：
4.4
作者：
EGERTON, M;SCOLLAY, R
通讯作者：
SCOLLAY, R
影响因子：
5.4
作者：
SCHONRICH, G;ALFERINK, J;ARNOLD, B
通讯作者：
ARNOLD, B
影响因子：
64.8
作者：
SCHORLE, H;HOLTSCHKE, T;HORAK, I
通讯作者：
HORAK, I