Evaluation of multiplexed cytokine and inflammation marker measurements: a methodologic study.
Evaluation of multiplexed cytokine and inflammation marker measurements: a methodologic study.
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DOI：
10.1158/1055-9965.epi-11-0221
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发表时间：
2011-09
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影响因子：
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通讯作者：
Hildesheim A
中科院分区：
文献类型：
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作者：
Chaturvedi AK;Kemp TJ;Pfeiffer RM;Biancotto A;Williams M;Munuo S;Purdue MP;Hsing AW;Pinto L;McCoy JP;Hildesheim A
慢性炎症在病因学上与几种癌症相关。我们评估了两种基于Luminex微珠的商业试剂盒和三种标本类型中116种炎症、免疫和代谢标志物的性能（检测高于检测下限的浓度的能力、变异系数[CV]和组内相关系数[ICC]）。从前列腺、肺、结肠直肠和卵巢癌试验中的100名无癌症参与者中，使用血清、肝素血浆和EDTA血浆样品。我们分别使用Bio-Rad和Millipore试剂盒测量了67和97种标志物的水平。对于每种标本类型，使用40个设盲重复样本（20个批内样本和20个跨批样本）评估重现性。在所有标本类型/试剂盒上，大多数标记物在>25%的个体中可检测到。在67种Bio-Rad标记物中，血清、肝素血浆和EDTA血浆中分别有51、52和47种标记物的跨批次CV <20%。同样，在97种Millipore标记物中，血清、肝素血浆和EDTA血浆中分别有75、69和78种标记物的跨批次CV <20%。当将样本类型的结果合并时，45种Bio-Rad和71种Millipore标记物具有可接受的性能（所有3种样本类型的可检测性>25%，3种样本类型中至少2种的跨批次CV <20%）。不同标本类型的中位浓度和ICC差异较小，Bio-Rad和Millipore之间差异较大。使用基于Luminex的多路复用方法，可以在血清和血浆样品中可靠地测量炎症和免疫标志物。多重测定可用于对炎症在癌症病因学中的作用进行流行病学调查。
Chronic inflammation is etiologically-related to several cancers. We evaluated the performance (ability to detect concentrations above the assay’s lower limit of detection, coefficients-of-variation [CVs], and intraclass correlation coefficients [ICCs]) of 116 inflammation, immune, and metabolic markers across two luminex bead-based commercial kits and three specimen types. From 100 cancer-free participants in the Prostate, Lung, Colorectal, and Ovarian Cancer Trial, serum, heparin plasma, and EDTA plasma samples were utilized. We measured levels of 67 and 97 markers using Bio-Rad and Millipore kits, respectively. Reproducibility was assessed using 40 blinded duplicates (20 within-batches and 20 across-batches) for each specimen type. A majority of markers were detectable in >25% of individuals on all specimen types/kits. Of the 67 Bio-Rad markers, 51, 52, and 47 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs <20%. Likewise, of 97 Millipore markers, 75, 69, and 78 markers in serum, heparin plasma, and EDTA plasma, respectively, had across-batch CVs <20%. When results were combined across specimen types, 45 Bio-Rad and 71 Millipore markers had acceptable performance (>25% detectability on all 3 specimen types and across-batch CVs <20% on at least 2 of 3 specimen types). Median concentrations and ICCs differed to a small extent across specimen types and to a large extent between Bio-Rad and Millipore. Inflammation and immune markers can be measured reliably in serum and plasma samples using multiplexed luminex-based methods. Multiplexed assays can be utilized for epidemiologic investigations into the role of inflammation in cancer etiology.