Genome organization and definition of the Penaeus monodon viral responsive protein 15 (PmVRP15) promoter.
Genome organization and definition of the Penaeus monodon viral responsive protein 15 (PmVRP15) promoter.
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DOI：
10.1016/j.fsi.2019.08.026
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发表时间：
2019-10
影响因子：
4.7
通讯作者：
Phattarunda Jaree;T. Kawai;C. Lo;A. Tassanakajon;Kunlaya Somboonwiwat
中科院分区：
文献类型：
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作者：
Phattarunda Jaree;T. Kawai;C. Lo;A. Tassanakajon;Kunlaya Somboonwiwat
斑节对虾病毒应答蛋白15（PmVRP15）是一个对白色斑点综合征病毒（WSSV）高度应答的基因。它是从血细胞中鉴定出来的，对WSSV的运输和组装起着重要作用。然而，目前对PmVRP15基因调控的研究还很有限。本研究对PmVRP15基因的基因组结构和5 ′上游启动子序列进行了研究。PmVRP15基因由4个外显子和3个内含子组成，起始密码子位于外显子2。根据5 ′上游序列确定了转录起始位点和TATA盒。通过缩小实验，确定5 ′上游启动子活性区位于核苷酸位置-525至+612。对转录因子结合位点的突变表明，干扰素调节因子（IRF）的结合位点（-495/-479）为阻遏物结合位点，而八聚体转录因子1（Oct-1）的结合位点（-275/-268）和活化T细胞核因子转录因子（NFAT）的结合位点（-228/-223）为激活物结合位点。这是首次报道可能在PmVRP15基因表达调控中起重要作用的转录因子。PmVRP15基因表达的调控机制尚需进一步研究。
The viral responsive protein 15 from the black tiger shrimpPenaeus monodon(PmVRP15) is a highly responsive gene upon white spot syndrome virus (WSSV) challenge. It is identified from hemocyte and important for WSSV trafficking and assembly. However, the knowledge ofPmVRP15gene regulation is limited. In the present study, the genome organization and 5′upstream promoter sequences ofPmVRP15gene were investigated. ThePmVRP15gene was found to contain 4 exons interrupted by 3 introns and the start codon was located in the exon 2. The transcription start site and TATA box were also determined from the 5′ upstream sequence. By using the narrow down experiment, the 5′ upstream promoter active region was determined to be at the nucleotide positions -525 to +612. Mutagenesis of the putative transcription factor (TF) binding sites revealed that the binding site of interferon regulatory factor (IRF) (-495/-479) was a repressor-binding site whereas those of the octamer transcription factor 1 (Oct-1) (-275/-268) and the nuclear factor of activated T-cells transcription factor (NFAT) (-228/-223) were activator-binding sites. This is the first report on the transcription factors that might play essential roles in modulating thePmVRP15gene expression. Nevertheless, the underlying regulation mechanism ofPmVRP15gene expression needs further investigation.