Manipulation and Measurement of AMPK Activity in Pancreatic Islets.

Manipulation and Measurement of AMPK Activity in Pancreatic Islets.
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胰岛 AMPK 活性的操作和测量。

DOI:
10.1007/978-1-4939-7598-3_26
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发表时间:
2018
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Martinez-Sanchez A
Martinez-Sanchez A
中科院分区:
--
文献类型:
--
作者:
Martinez-Sanchez A

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能量传感器AMPK激活的蛋白激酶(AMPK)在胰岛素分泌β细胞中的作用仍然不清楚,也是一个深入研究的主题。在本章中,我们的目的是提供一个详细的描述的方法,我们的小组经常适用于在小鼠和人类胰岛AMPK功能的研究。因此,我们提供了详细的协议,以分离和/或培养小鼠和人类胰岛,调节和测量AMPK活性在分离的胰岛,并评估其对胰岛功能的影响。
The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.
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