OPSONIC REQUIREMENTS FOR THE UPTAKE OF CRYPTOCOCCUS-NEOFORMANS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES
OPSONIC REQUIREMENTS FOR THE UPTAKE OF CRYPTOCOCCUS-NEOFORMANS BY HUMAN POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES
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DOI：
10.1093/infdis/145.6.870
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发表时间：
1982-01-01
影响因子：
6.4
通讯作者：
REPINE, JE
中科院分区：
文献类型：
--
作者：
DAVIES, SF;CLIFFORD, DP;REPINE, JE
应用化学发光法测定新生隐球菌与人外周血多形核白细胞（PMNLs）和单核细胞（MNs）相互作用的调理素需求量。PMNLs和MN的峰值化学发光（平均值±SD）分别为172 ± 25 x 103和50 ± 13 x 103（含20%混合正常人血清），而PMNLs和MN的峰值化学发光（平均值±SD）分别为10 ± 3 x 103和6 ± 1 x 103（不含血清）（每次比较P< 0.005）。研究了血清需求的性质;需要替代途径血清补体成分。旁路途径被C直接激活。neoformans不需要特异性抗体。化学发光法提供了一种定量和可重复的测定人吞噬细胞与C. neoformans和可用于研究其他真菌的调理素需求。
Chemiluminescence was used to determine opsonic requirements for interaction ofCryptococcus neoformanswith human peripheral blood polymorphonuclear leukocytes (PMNLs) and monocytes (MNs). Peak chemiluminescence (mean ±SD) was 172 ± 25 x 103for PMNLs and 50 ± 13 x 103for MNs with 20% pooled normal human serum vs. 10 ± 3 x 103for PMNLs and 6 ± 1 x 103for MNs without serum (P< 0.005 for each comparison). The nature of the serum requirement was investigated; alternative pathway serum complement components were necessary. The alternative pathway was activated directly byC. neoformanswithout the need for specific antibody. Chemiluminescence provided a quantitative and reproducible assay of the interaction of human phagocytes withC. neoformansand may be useful in studying opsonic requirements of other fungi.